{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(1)"],"submitter":["Zhu D"],"pubmed_abstract":["Relative to the 300-kV accelerating field, electrons accelerated under lower voltages are potentially scattered more strongly. Lowering the accelerate voltage has been suggested to enhance the signal-to-noise ratio (SNR) of cryo-electron microscopy (cryo-EM) images of small-molecular-weight proteins (<100 kD). However, the detection efficient of current Direct Detection Devices (DDDs) and temporal coherence of cryo-EM decrease at lower voltage, leading to loss of SNR. Here, we present an electron counting algorithm to improve the detection of low-energy electrons. The counting algorithm increased the SNR of 120-kV and 200-kV cryo-EM image from a Falcon III camera by 8%, 20% at half the Nyquist frequency and 21%, 80% at Nyquist frequency, respectively, resulting in a considerable improvemen"],"journal":["Communications biology"],"pagination":["321"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8987035"],"repository":["biostudies-literature"],"pubmed_title":["An electron counting algorithm improves imaging of proteins with low-acceleration-voltage cryo-electron microscope."],"pmcid":["PMC8987035"],"pubmed_authors":["Zhang X","Wu C","Zhu D","Shi H"],"additional_accession":[]},"is_claimable":false,"name":"An electron counting algorithm improves imaging of proteins with low-acceleration-voltage cryo-electron microscope.","description":"Relative to the 300-kV accelerating field, electrons accelerated under lower voltages are potentially scattered more strongly. Lowering the accelerate voltage has been suggested to enhance the signal-to-noise ratio (SNR) of cryo-electron microscopy (cryo-EM) images of small-molecular-weight proteins (<100 kD). However, the detection efficient of current Direct Detection Devices (DDDs) and temporal coherence of cryo-EM decrease at lower voltage, leading to loss of SNR. Here, we present an electron counting algorithm to improve the detection of low-energy electrons. The counting algorithm increased the SNR of 120-kV and 200-kV cryo-EM image from a Falcon III camera by 8%, 20% at half the Nyquist frequency and 21%, 80% at Nyquist frequency, respectively, resulting in a considerable improvemen","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-21T16:50:57.665Z","creation":"2025-04-21T16:50:57.665Z"},"accession":"S-EPMC8987035","cross_references":{"pubmed":["35388174"],"doi":["10.1038/s42003-022-03284-1"]}}