biostudies-literatureLillis PEScience Foundation IrelandNational University of Ireland, Maynoothe0266164https://www.ebi.ac.uk/biostudies/studies/S-EPMC8989221biostudies-literatureUnknown17(4)Entomopathogenic nematodes (EPN) of the genera Steinernema and Heterorhabditis are parasites which kill and reproduce within insects. While both have life cycles centred around their developmentally arrested, nonfeeding and stress tolerant infective juvenile (IJ) stage, they are relatively distantly related. These IJs are promising biocontrol agents, and their shelf life and stress tolerance may be enhanced by storage at low temperatures. The purpose of this study was to investigate how the proteome of the IJs of two distantly related EPN species is affected by storage at 9°C (for up to 9 weeks) and 20°C (for up to 6 weeks), using label-free quantitative proteomics. Overall, more proteins were detected in S. carpocapsae (2422) than in H. megidis (1582). The S. carpocapsae proteome was stroPloS oneThe effect of temperature conditioning (9°C and 20°C) on the proteome of entomopathogenic nematode infective juveniles.PMC898922112/RI/2346(3)Griffin CTLillis PECarolan JCfalseThe effect of temperature conditioning (9°C and 20°C) on the proteome of entomopathogenic nematode infective juveniles.Entomopathogenic nematodes (EPN) of the genera Steinernema and Heterorhabditis are parasites which kill and reproduce within insects. While both have life cycles centred around their developmentally arrested, nonfeeding and stress tolerant infective juvenile (IJ) stage, they are relatively distantly related. These IJs are promising biocontrol agents, and their shelf life and stress tolerance may be enhanced by storage at low temperatures. The purpose of this study was to investigate how the proteome of the IJs of two distantly related EPN species is affected by storage at 9°C (for up to 9 weeks) and 20°C (for up to 6 weeks), using label-free quantitative proteomics. Overall, more proteins were detected in S. carpocapsae (2422) than in H. megidis (1582). The S. carpocapsae proteome was stro2022-01-01T00:00:00Z20222026-04-08T17:58:01.66Z2025-04-06T21:54:26.268ZS-EPMC89892213539003410.1371/journal.pone.0266164