{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang Y"],"funding":["Natural Science Foundation of China"],"pagination":["838698"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8989280"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["<i>Escherichia coli recA</i> <sup>-</sup> strains are usually used for cloning to prevent insert instability <i>via</i> RecA-dependent recombination. Here, we report that <i>E. coli</i> BW25113 (<i>recA</i> <sup>+</sup>) competent cells prepared by using a previously reported transformation and storage solution (TSS) had 100-fold or higher transformation efficiency than the commonly used <i>E. coli</i> cloning strains, including XL1-Blue MRF'. The cloning success rates with <i>E. coli</i> BW25113 were 440 to 1,267-fold higher than those with <i>E. coli</i> XL1-Blue MRF' when several inserts were assembled into four vectors by using a simple DNA assembly method. The difference was in part due to RecA, as the <i>recA</i> deletion in <i>E. coli</i> BW25113 reduced the transformation efficienc"],"journal":["Frontiers in microbiology"],"pubmed_title":["<i>Escherichia coli</i> BW25113 Competent Cells Prepared Using a Simple Chemical Method Have Unmatched Transformation and Cloning Efficiencies."],"pmcid":["PMC8989280"],"funding_grant_id":["31870085","31961133015","91951202"],"pubmed_authors":["Wang M","Liu H","Xia Y","Yang Y","Xun L","Zhao R","Yu Q"],"additional_accession":[]},"is_claimable":false,"name":"<i>Escherichia coli</i> BW25113 Competent Cells Prepared Using a Simple Chemical Method Have Unmatched Transformation and Cloning Efficiencies.","description":"<i>Escherichia coli recA</i> <sup>-</sup> strains are usually used for cloning to prevent insert instability <i>via</i> RecA-dependent recombination. Here, we report that <i>E. coli</i> BW25113 (<i>recA</i> <sup>+</sup>) competent cells prepared by using a previously reported transformation and storage solution (TSS) had 100-fold or higher transformation efficiency than the commonly used <i>E. coli</i> cloning strains, including XL1-Blue MRF'. The cloning success rates with <i>E. coli</i> BW25113 were 440 to 1,267-fold higher than those with <i>E. coli</i> XL1-Blue MRF' when several inserts were assembled into four vectors by using a simple DNA assembly method. The difference was in part due to RecA, as the <i>recA</i> deletion in <i>E. coli</i> BW25113 reduced the transformation efficienc","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-05T00:27:38.935Z","creation":"2025-04-05T00:27:38.935Z"},"accession":"S-EPMC8989280","cross_references":{"pubmed":["35401484"],"doi":["10.3389/fmicb.2022.838698"]}}