{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Leong KW"],"funding":["Dana-Farber Cancer Institute","NCI NIH HHS","National Institutes of Health"],"pagination":["e32"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8989544"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(6)"],"pubmed_abstract":["Detection of low-level DNA mutations can reveal recurrent, hotspot genetic changes of clinical relevance to cancer, prenatal diagnostics, organ transplantation or infectious diseases. However, the high excess of wild-type (WT) alleles, which are concurrently present, often hinders identification of salient genetic changes. Here, we introduce UV-mediated cross-linking minor allele enrichment (UVME), a novel approach that incorporates ultraviolet irradiation (∼365 nm UV) DNA cross-linking either before or during PCR amplification. Oligonucleotide probes matching the WT target sequence and incorporating a UV-sensitive 3-cyanovinylcarbazole nucleoside modification are employed for cross-linking WT DNA. Mismatches formed with mutated alleles reduce DNA binding and UV-mediated cross-linking and "],"journal":["Nucleic acids research"],"pubmed_title":["Mutation enrichment in human DNA samples via UV-mediated cross-linking."],"pmcid":["PMC8989544"],"funding_grant_id":["R01 CA221874"],"pubmed_authors":["Yu F","Leong KW","Makrigiorgos GM"],"additional_accession":[]},"is_claimable":false,"name":"Mutation enrichment in human DNA samples via UV-mediated cross-linking.","description":"Detection of low-level DNA mutations can reveal recurrent, hotspot genetic changes of clinical relevance to cancer, prenatal diagnostics, organ transplantation or infectious diseases. However, the high excess of wild-type (WT) alleles, which are concurrently present, often hinders identification of salient genetic changes. Here, we introduce UV-mediated cross-linking minor allele enrichment (UVME), a novel approach that incorporates ultraviolet irradiation (∼365 nm UV) DNA cross-linking either before or during PCR amplification. Oligonucleotide probes matching the WT target sequence and incorporating a UV-sensitive 3-cyanovinylcarbazole nucleoside modification are employed for cross-linking WT DNA. Mismatches formed with mutated alleles reduce DNA binding and UV-mediated cross-linking and ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-03-27T15:18:01.904Z","creation":"2025-04-04T19:10:47.273Z"},"accession":"S-EPMC8989544","cross_references":{"pubmed":["34904676"],"doi":["10.1093/nar/gkab1222"]}}