biostudies-literatureUnknown13(6)Liu WThe eukaryotic chaperonin family is vital for cell survival. The dysregulation of chaperonin-containing TCP-1 subunit 3 (CCT3) is implicated in several types of malignant tumors' development. However, its functional role in melanoma remains unknown. Here we elucidate the functional contribution to CCT3 to melanoma progression. The results indicated that CCT3 highly expressed in melanoma tissues, and CCT3 overexpression is correlated with clinical stage in melanoma patients. Knockdown of CCT3 by shRNA in melanoma cells inhibited cell proliferation and cell cycle progression and induced cell apoptosis <i>in vitro</i>. <i>In vivo</i>, tumor growth in the nude mice was significantly inhibited after CCT3 silencing. Importantly, the gene array analysis showed that CCT3 depletions inhibited cycliJournal of Cancer1958-1971https://www.ebi.ac.uk/biostudies/studies/S-EPMC8990421biostudies-literatureSuppression of CCT3 inhibits melanoma cell proliferation by downregulating CDK1 expression.PMC8990421Liu YLi YJiang GZhang XHan ZChen CYang CLiu WfalseSuppression of CCT3 inhibits melanoma cell proliferation by downregulating CDK1 expression.The eukaryotic chaperonin family is vital for cell survival. The dysregulation of chaperonin-containing TCP-1 subunit 3 (CCT3) is implicated in several types of malignant tumors' development. However, its functional role in melanoma remains unknown. Here we elucidate the functional contribution to CCT3 to melanoma progression. The results indicated that CCT3 highly expressed in melanoma tissues, and CCT3 overexpression is correlated with clinical stage in melanoma patients. Knockdown of CCT3 by shRNA in melanoma cells inhibited cell proliferation and cell cycle progression and induced cell apoptosis <i>in vitro</i>. <i>In vivo</i>, tumor growth in the nude mice was significantly inhibited after CCT3 silencing. Importantly, the gene array analysis showed that CCT3 depletions inhibited cycli2022-01-01T00:00:00Z20222026-05-31T07:14:10.481Z2025-04-04T07:56:07.301ZS-EPMC89904213539972210.7150/jca.69497