biostudies-literatureSouza MCCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFundação de Amparo à Pesquisa do Estado de São Paulo100008https://www.ebi.ac.uk/biostudies/studies/S-EPMC8991615biostudies-literatureUnknown2The in-line coupling of the pressurized liquid extraction with a solid-phase adsorbent and a UV-Vis detector for the simultaneous extraction and separation of bioactive compounds from yerba mate (PLE-SPE-UV) was carried out in two stages. In the first stage, water was used as a solvent, while in the second stage, ethanol was used. For the optimization of the method, different adsorbents (Sepra C18-E, Isolute C18-EC, and Strata-X C18), temperatures (40-80 °C), solvent flow-rate (1-3 mL/min), and pH (4.0 and 8.0) were evaluated. By using a UV-Vis detector on-line, it is possible to monitor the process in real-time. The developed method allowed obtaining similar or higher recoveries of all the compounds classes than other methods, such as ultrasound-assisted extraction, stirring, maceration, Food chemistry. Molecular sciencesSimultaneous extraction and separation of compounds from mate (<i>Ilex paraguariensis</i>) leaves by pressurized liquid extraction coupled with solid-phase extraction and in-line UV detection.PMC899161588887.310558/2018-002018/14582-5Silva LCSanches VLSalvador MPChaves JOda Cunha DTSouza MCFoster Carneiro TRostagno MAfalseSimultaneous extraction and separation of compounds from mate (<i>Ilex paraguariensis</i>) leaves by pressurized liquid extraction coupled with solid-phase extraction and in-line UV detection.The in-line coupling of the pressurized liquid extraction with a solid-phase adsorbent and a UV-Vis detector for the simultaneous extraction and separation of bioactive compounds from yerba mate (PLE-SPE-UV) was carried out in two stages. In the first stage, water was used as a solvent, while in the second stage, ethanol was used. For the optimization of the method, different adsorbents (Sepra C18-E, Isolute C18-EC, and Strata-X C18), temperatures (40-80 °C), solvent flow-rate (1-3 mL/min), and pH (4.0 and 8.0) were evaluated. By using a UV-Vis detector on-line, it is possible to monitor the process in real-time. The developed method allowed obtaining similar or higher recoveries of all the compounds classes than other methods, such as ultrasound-assisted extraction, stirring, maceration, 2021-01-01T00:00:00Z2021 Jul2025-04-04T22:17:55.082Z2025-04-04T22:17:55.082ZS-EPMC89916153541563810.1016/j.fochms.2020.100008