biostudies-literatureUnknown22(1)Sivapalan L<h4>Background</h4>The utility of circulating tumour DNA (ctDNA) for longitudinal tumour monitoring in pancreatic ductal adenocarcinoma (PDAC) has not been explored beyond mutations in the KRAS proto-oncogene. Here, we aimed to characterise and track patient-specific somatic ctDNA variants, to assess longitudinal changes in disease burden and explore the landscape of actionable alterations.<h4>Methods</h4>We followed 3 patients with resectable disease and 4 patients with unresectable disease, including 4 patients with ≥ 3 serial follow-up samples, of whom 2 were rare long survivors (> 5 years). We performed whole exome sequencing of tumour gDNA and plasma ctDNA (n = 20) collected over a ~ 2-year period from diagnosis through treatment to death or final follow-up. Plasma from 3 chronic pancBMC cancer369https://www.ebi.ac.uk/biostudies/studies/S-EPMC8991893biostudies-literatureLongitudinal profiling of circulating tumour DNA for tracking tumour dynamics in pancreatic cancer.PMC8991893Chelala CSivapalan LThorn GJRoss-Adams HKocher HMGadaleta EfalseLongitudinal profiling of circulating tumour DNA for tracking tumour dynamics in pancreatic cancer.<h4>Background</h4>The utility of circulating tumour DNA (ctDNA) for longitudinal tumour monitoring in pancreatic ductal adenocarcinoma (PDAC) has not been explored beyond mutations in the KRAS proto-oncogene. Here, we aimed to characterise and track patient-specific somatic ctDNA variants, to assess longitudinal changes in disease burden and explore the landscape of actionable alterations.<h4>Methods</h4>We followed 3 patients with resectable disease and 4 patients with unresectable disease, including 4 patients with ≥ 3 serial follow-up samples, of whom 2 were rare long survivors (> 5 years). We performed whole exome sequencing of tumour gDNA and plasma ctDNA (n = 20) collected over a ~ 2-year period from diagnosis through treatment to death or final follow-up. Plasma from 3 chronic panc2022-01-01T00:00:00Z2022 Apr2025-04-26T15:16:45.228Z2025-04-06T14:50:32.593ZS-EPMC89918933539285410.1186/s12885-022-09387-6