{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Alcaman-Arias ME"],"pubmed_abstract":["Marine ammonia oxidizers that oxidize ammonium to nitrite are abundant in polar waters, especially during the winter in the deeper mixed-layer of West Antarctic Peninsula (WAP) waters. However, the activity and abundance of ammonia-oxidizers during the summer in surface coastal Antarctic waters remain unclear. In this study, the ammonia-oxidation rates, abundance and identity of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were evaluated in the marine surface layer (to 30 m depth) in Chile Bay (Greenwich Island, WAP) over three consecutive late-summer periods (2017, 2018, and 2019). Ammonia-oxidation rates of 68.31 nmol N L<sup>-1</sup> day<sup>-1</sup> (2018) and 37.28 nmol N L<sup>-1</sup> day<sup>-1</sup> (2019) were detected from illuminated 2 m seawater incubations. However, high ammonia-oxidation rates between 267.75 and 109.38 nmol N L<sup>-1</sup> day<sup>-1</sup> were obtained under the dark condition at 30 m in 2018 and 2019, respectively. During the late-summer sampling periods both stratifying and mixing events occurring in the water column over short timescales (February-March). Metagenomic analysis of seven nitrogen cycle modules revealed the presence of ammonia-oxidizers, such as the Archaea <i>Nitrosopumilus</i> and the Bacteria <i>Nitrosomonas</i> and <i>Nitrosospira</i>, with AOA often being more abundant than AOB. However, quantification of specific <i>amo</i>A gene transcripts showed number of AOB being two orders of magnitude higher than AOA, with <i>Nitrosomonas</i> representing the most transcriptionally active AOB in the surface waters. Additionally, <i>Candidatus</i> Nitrosopelagicus and <i>Nitrosopumilus</i>, phylogenetically related to surface members of the NP-ε and NP-γ clades respectively, were the predominant AOA. Our findings expand the known distribution of ammonium-oxidizers to the marine surface layer, exposing their potential ecological role in supporting the marine Antarctic system during the productive summer periods."],"journal":["Frontiers in microbiology"],"pagination":["821902"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8992545"],"repository":["biostudies-literature"],"pubmed_title":["Surface Ammonia-Oxidizer Abundance During the Late Summer in the West Antarctic Coastal System."],"pmcid":["PMC8992545"],"pubmed_authors":["Cifuentes-Anticevic J","Troncoso M","Bello E","Testa G","Alcaman-Arias ME","Diez B","Farias L"],"additional_accession":[]},"is_claimable":false,"name":"Surface Ammonia-Oxidizer Abundance During the Late Summer in the West Antarctic Coastal System.","description":"Marine ammonia oxidizers that oxidize ammonium to nitrite are abundant in polar waters, especially during the winter in the deeper mixed-layer of West Antarctic Peninsula (WAP) waters. However, the activity and abundance of ammonia-oxidizers during the summer in surface coastal Antarctic waters remain unclear. In this study, the ammonia-oxidation rates, abundance and identity of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were evaluated in the marine surface layer (to 30 m depth) in Chile Bay (Greenwich Island, WAP) over three consecutive late-summer periods (2017, 2018, and 2019). Ammonia-oxidation rates of 68.31 nmol N L<sup>-1</sup> day<sup>-1</sup> (2018) and 37.28 nmol N L<sup>-1</sup> day<sup>-1</sup> (2019) were detected from illuminated 2 m seawater incubations. However, high ammonia-oxidation rates between 267.75 and 109.38 nmol N L<sup>-1</sup> day<sup>-1</sup> were obtained under the dark condition at 30 m in 2018 and 2019, respectively. During the late-summer sampling periods both stratifying and mixing events occurring in the water column over short timescales (February-March). Metagenomic analysis of seven nitrogen cycle modules revealed the presence of ammonia-oxidizers, such as the Archaea <i>Nitrosopumilus</i> and the Bacteria <i>Nitrosomonas</i> and <i>Nitrosospira</i>, with AOA often being more abundant than AOB. However, quantification of specific <i>amo</i>A gene transcripts showed number of AOB being two orders of magnitude higher than AOA, with <i>Nitrosomonas</i> representing the most transcriptionally active AOB in the surface waters. Additionally, <i>Candidatus</i> Nitrosopelagicus and <i>Nitrosopumilus</i>, phylogenetically related to surface members of the NP-ε and NP-γ clades respectively, were the predominant AOA. Our findings expand the known distribution of ammonium-oxidizers to the marine surface layer, exposing their potential ecological role in supporting the marine Antarctic system during the productive summer periods.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T17:26:41.061Z","creation":"2025-04-04T23:03:10.643Z"},"accession":"S-EPMC8992545","cross_references":{"pubmed":["35401462"],"doi":["10.3389/fmicb.2022.821902"]}}