biostudies-literatureLiu TNational Natural Science Foundation of China842893https://www.ebi.ac.uk/biostudies/studies/S-EPMC8993504biostudies-literatureUnknown13<i>Gastrodia elata</i> is an entirely heterotrophic plant, the growth of which is completely reliant on <i>Armillaria gallica</i>, an orchid mycorrhizal fungus. To avoid damaging ecosystems, <i>G. elata</i> cultivation is shifting from woodland to farmland. However, whether the microbial community structure remains stable during this conversation is unknown. Here, we cultivated <i>G. elata</i> in woodland or farmland and found that woodland-cultivated <i>G. elata</i> produced a greater yield and larger tuber size. The relative abundance of <i>Rahnella</i> was 22.84- and 122.25-fold higher in woodland- and farmland-cultivated soil samples, respectively, than that in uncultivated soil samples. To investigate how <i>Rahnella</i> impacts the growth of <i>G. elata</i> and establishes symbiosis Frontiers in microbiologyMycorrhizosphere Bacteria, <i>Rahnella</i> sp. HPDA25, Promotes the Growth of <i>Armillaria gallica</i> and Its Parasitic Host <i>Gastrodia elata</i>.PMC899350481891013Han PLi XHua ZHuang LLiu TJin YZhao YZhou JYuan YfalseMycorrhizosphere Bacteria, <i>Rahnella</i> sp. HPDA25, Promotes the Growth of <i>Armillaria gallica</i> and Its Parasitic Host <i>Gastrodia elata</i>.<i>Gastrodia elata</i> is an entirely heterotrophic plant, the growth of which is completely reliant on <i>Armillaria gallica</i>, an orchid mycorrhizal fungus. To avoid damaging ecosystems, <i>G. elata</i> cultivation is shifting from woodland to farmland. However, whether the microbial community structure remains stable during this conversation is unknown. Here, we cultivated <i>G. elata</i> in woodland or farmland and found that woodland-cultivated <i>G. elata</i> produced a greater yield and larger tuber size. The relative abundance of <i>Rahnella</i> was 22.84- and 122.25-fold higher in woodland- and farmland-cultivated soil samples, respectively, than that in uncultivated soil samples. To investigate how <i>Rahnella</i> impacts the growth of <i>G. elata</i> and establishes symbiosis 2022-01-01T00:00:00Z20222025-04-19T00:39:56.399Z2025-04-07T11:42:16.807ZS-EPMC89935043540148010.3389/fmicb.2022.842893