biostudies-literatureUnknown8(1)Luo HThe underlying mechanisms of methionine adenosyltransferase 2 A (MAT2A)-mediated cervical cancer progression under nutrient stress are largely elusive. Therefore, our study aims to investigate molecular mechanism by which MAT2A-indcued cervical oncogenesis. The interaction between MAT2A and programmed cell death protein 6 (PDCD6) in cervical cancer cell lines was detected by immunoprecipitation, immunoblotting and mass spectrometric analysis. A panel of inhibitors that are linked to stress responsive kinases were utilized to detect related pathways by immunoblotting. Cell proliferation and apoptosis were investigated by CCK-8 and flow cytometry. Apoptosis related protein level of Bcl-2, Bax and Caspase-3 was also analyzed in cells with PDCD6 K90 methylation mutation. The association betweeCell death discovery176https://www.ebi.ac.uk/biostudies/studies/S-EPMC8993843biostudies-literatureMAT2A facilitates PDCD6 methylation and promotes cell growth under glucose deprivation in cervical cancer.PMC8993843Liu YChen FZhu XLuo HWang ZZhang JASong YfalseMAT2A facilitates PDCD6 methylation and promotes cell growth under glucose deprivation in cervical cancer.The underlying mechanisms of methionine adenosyltransferase 2 A (MAT2A)-mediated cervical cancer progression under nutrient stress are largely elusive. Therefore, our study aims to investigate molecular mechanism by which MAT2A-indcued cervical oncogenesis. The interaction between MAT2A and programmed cell death protein 6 (PDCD6) in cervical cancer cell lines was detected by immunoprecipitation, immunoblotting and mass spectrometric analysis. A panel of inhibitors that are linked to stress responsive kinases were utilized to detect related pathways by immunoblotting. Cell proliferation and apoptosis were investigated by CCK-8 and flow cytometry. Apoptosis related protein level of Bcl-2, Bax and Caspase-3 was also analyzed in cells with PDCD6 K90 methylation mutation. The association betwee2022-01-01T00:00:00Z2022 Apr2025-04-04T22:16:53.443Z2025-04-04T22:16:53.443ZS-EPMC89938433539651210.1038/s41420-022-00987-6