biostudies-literaturePrem SBundesministerium für Bildung und ForschungFreie Universität Berlin58https://www.ebi.ac.uk/biostudies/studies/S-EPMC8994360biostudies-literatureUnknown21(1)Fatty acid hydratases are unique to microorganisms. Their native function is the oxidation of unsaturated C-C bonds to enable detoxification of environmental toxins. Within this enzyme family, the oleate hydratases (Ohys), which catalyze the hydroxylation of oleic acid to 10-(R)-hydroxy stearic acid (10-HSA) have recently gained particular industrial interest. 10-HSA is considered to be a replacement for 12-(R)-hydroxy stearic acid (12-HSA), which has a broad application in the chemical and pharmaceutical industry. As 12-HSA is obtained through an energy consuming synthesis process, the biotechnological route for sustainable 10-HSA production is of significant industrial interest. All Ohys identified to date have a non-redox active FAD bound in their active site. Ohys can be divided in sevMicrobial cell factoriesTowards an understanding of oleate hydratases and their application in industrial processes.PMC899436003SF0577A031B0853Helmer CPODimos NBruck TPrem SGarbe DLoll BHimpich SfalseTowards an understanding of oleate hydratases and their application in industrial processes.Fatty acid hydratases are unique to microorganisms. Their native function is the oxidation of unsaturated C-C bonds to enable detoxification of environmental toxins. Within this enzyme family, the oleate hydratases (Ohys), which catalyze the hydroxylation of oleic acid to 10-(R)-hydroxy stearic acid (10-HSA) have recently gained particular industrial interest. 10-HSA is considered to be a replacement for 12-(R)-hydroxy stearic acid (12-HSA), which has a broad application in the chemical and pharmaceutical industry. As 12-HSA is obtained through an energy consuming synthesis process, the biotechnological route for sustainable 10-HSA production is of significant industrial interest. All Ohys identified to date have a non-redox active FAD bound in their active site. Ohys can be divided in sev2022-01-01T00:00:00Z2022 Apr2026-05-31T21:04:25.252Z2025-04-04T19:30:29.025ZS-EPMC89943603539758510.1186/s12934-022-01777-6