{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou F"],"funding":["Scientific Research Project of Shanghai Municipal Health and Family Planning Commission"],"pagination":["e12629"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8995023"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10"],"pubmed_abstract":["<h4>Background</h4>Whether circRAN, which acts as a microRNA sponge, plays a role in 5-fluorouracil (5-Fu) resistant gastric cancer has not been reported. In this study, a 5-Fu resistant cell line with an IC50 of 16.59 µM was constructed.<h4>Methods</h4>Using comparative analysis of circRNA in the transcriptomics of resistant and sensitive strains, 31 differentially expressed circRNAs were detected, and the microRNA interacting with them was predicted.<h4>Results</h4>Hsacirc_004413 was selected for verification in drug resistant and sensitive cells. By interfering with hsacirc_004413 using antisense RNA, the sensitivity of drug resistant cells to 5-Fu was significantly promoted, and the apoptosis and necrosis of the cells were significantly increased. In sensitive cells, inhibition by inhibitors enhanced the resistance of cells to 5-Fu. We hypothesize that hsacirc_004413 makes gastric cancer cells resistant to 5-Fu mainly through adsorption of miR-145-5p."],"journal":["PeerJ"],"pubmed_title":["The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p."],"pmcid":["PMC8995023"],"funding_grant_id":["201640310"],"pubmed_authors":["Ding W","Chen J","Huang J","Mao Q","Jiang X","Zhou F","Sun X","Xu W","Zhong L","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p.","description":"<h4>Background</h4>Whether circRAN, which acts as a microRNA sponge, plays a role in 5-fluorouracil (5-Fu) resistant gastric cancer has not been reported. In this study, a 5-Fu resistant cell line with an IC50 of 16.59 µM was constructed.<h4>Methods</h4>Using comparative analysis of circRNA in the transcriptomics of resistant and sensitive strains, 31 differentially expressed circRNAs were detected, and the microRNA interacting with them was predicted.<h4>Results</h4>Hsacirc_004413 was selected for verification in drug resistant and sensitive cells. By interfering with hsacirc_004413 using antisense RNA, the sensitivity of drug resistant cells to 5-Fu was significantly promoted, and the apoptosis and necrosis of the cells were significantly increased. In sensitive cells, inhibition by inhibitors enhanced the resistance of cells to 5-Fu. We hypothesize that hsacirc_004413 makes gastric cancer cells resistant to 5-Fu mainly through adsorption of miR-145-5p.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-31T21:03:45.327Z","creation":"2025-02-19T04:23:04.055Z"},"accession":"S-EPMC8995023","cross_references":{"pubmed":["35415017"],"doi":["10.7717/peerj.12629"]}}