biostudies-literatureTran ANational Institute of Allergy and Infectious DiseasesNational Center for Advancing Translational SciencesNCATS NIH HHSNIAID NIH HHSUniversity of MarylandSchool of Pharmacy, University of MarylandAmerican Society of Mass SpectrometryNIGMS NIH HHS2322-2333https://www.ebi.ac.uk/biostudies/studies/S-EPMC8995026biostudies-literatureUnknown32(9)There is an unmet need to develop analytical strategies that not only characterize the lipid composition of the viral envelope but also do so on a time scale that would allow for high-throughput analysis. With that in mind, we report the use of atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) high-resolution mass spectrometry (HRMS) combined with lithium adduct consolidation to profile total lipid extracts rapidly and confidently from enveloped viruses. The use of AP-MALDI reduced the dependency of using a dedicated MALDI mass spectrometer and allowed for interfacing the MALDI source to a mass spectrometer with the desired features, which included high mass resolving power (>100000) and tandem mass spectrometry. AP-MALDI combined with an optimized MALDI matrix Journal of the American Society for Mass SpectrometryRapid Detection of Viral Envelope Lipids Using Lithium Adducts and AP-MALDI High-Resolution Mass Spectrometry.PMC8995026UL1 TR003098SOP1841-IQB2014R01 IA109022R21 AI1423771UL1TR003098T32 GM066706R01 AI109022Monreal IAAguilar HCJones JWTran AMoskovets EfalseRapid Detection of Viral Envelope Lipids Using Lithium Adducts and AP-MALDI High-Resolution Mass Spectrometry.There is an unmet need to develop analytical strategies that not only characterize the lipid composition of the viral envelope but also do so on a time scale that would allow for high-throughput analysis. With that in mind, we report the use of atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) high-resolution mass spectrometry (HRMS) combined with lithium adduct consolidation to profile total lipid extracts rapidly and confidently from enveloped viruses. The use of AP-MALDI reduced the dependency of using a dedicated MALDI mass spectrometer and allowed for interfacing the MALDI source to a mass spectrometer with the desired features, which included high mass resolving power (>100000) and tandem mass spectrometry. AP-MALDI combined with an optimized MALDI matrix 2021-01-01T00:00:00Z2021 Sep2025-04-19T12:55:00.987Z2025-04-19T12:55:00.987ZS-EPMC89950263388629410.1021/jasms.1c00058