biostudies-literatureUnknownGiroux NSNIAID NIH HHSNHLBI NIH HHSNIGMS NIH HHSSARS-CoV-2 infection triggers profound and variable immune responses in human hosts. Chromatin remodeling has been observed in individuals severely ill or convalescing with COVID-19, but chromatin remodeling early in disease prior to anti-spike protein IgG seroconversion has not been defined. We performed the Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) and RNA-seq on peripheral blood mononuclear cells (PBMCs) from outpatients with mild or moderate symptom severity at different stages of clinical illness. Early in the disease course prior to IgG seroconversion, modifications in chromatin accessibility associate with mild or moderate symptoms are already robust and include severity-associated changes in accessibility of genes in interleukin signaling, regulation ofResearch squarers.3.rs-1479864https://www.ebi.ac.uk/biostudies/studies/S-EPMC8996625biostudies-literatureDifferential chromatin accessibility in peripheral blood mononuclear cells underlies COVID-19 disease severity prior to seroconversion.PMC8996625R35 GM122465K08 HL130557UC6 AI05860775N93019C00015Satterwhite LLRivera GOBose SHenao RGinsburg GSTsalik ELWang ENicholson BPXi RDe Ussel MIBurke TWChung HASempowski GDGiroux NSKraft BDDing SKo ERWoods CPetzold ERotstein TMcClain MTDenny TNShen XChen TfalseDifferential chromatin accessibility in peripheral blood mononuclear cells underlies COVID-19 disease severity prior to seroconversion.SARS-CoV-2 infection triggers profound and variable immune responses in human hosts. Chromatin remodeling has been observed in individuals severely ill or convalescing with COVID-19, but chromatin remodeling early in disease prior to anti-spike protein IgG seroconversion has not been defined. We performed the Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) and RNA-seq on peripheral blood mononuclear cells (PBMCs) from outpatients with mild or moderate symptom severity at different stages of clinical illness. Early in the disease course prior to IgG seroconversion, modifications in chromatin accessibility associate with mild or moderate symptoms are already robust and include severity-associated changes in accessibility of genes in interleukin signaling, regulation of2022-01-01T00:00:00Z2022 Apr2025-04-04T08:52:59.458Z2025-04-04T08:52:59.458ZS-EPMC89966253541134310.21203/rs.3.rs-1479864/v1