{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(7)"],"submitter":["Padmathilake KRE"],"pubmed_abstract":["<i>Leptosphaeria maculans</i> causes blackleg disease, which is one of the most destructive diseases of canola (<i>Brassica napus</i> L.). Due to the erosion of the current resistance in <i>B. napus</i>, it is pivotal to introduce new resistant genotypes to the growers. This study evaluated the potential of <i>Rlm7</i> gene as resistance to its corresponding avirulence <i>AvrLm7</i> gene is abundant. The <i>Rlm7</i> line was inoculated with <i>L. maculans</i> isolate with <i>AvrLm7</i>; UMAvr7; and the CRISPR/Cas9 knockout <i>AvrLm7</i> mutant, umavr7, of the same isolate to cause incompatible and compatible interactions, respectively. Dual RNA-seq showed differential gene expressions in both interactions. High expressions of virulence-related pathogen genes-CAZymes, merops, and effector p"],"journal":["International journal of molecular sciences"],"pagination":["3964"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8999614"],"repository":["biostudies-literature"],"pubmed_title":["<i>Leptosphaeria maculans</i>-<i>Brassica napus</i> Battle: A Comparison of Incompatible vs. Compatible Interactions Using Dual RNASeq."],"pmcid":["PMC8999614"],"pubmed_authors":["Padmathilake KRE","Fernando WGD"],"additional_accession":[]},"is_claimable":false,"name":"<i>Leptosphaeria maculans</i>-<i>Brassica napus</i> Battle: A Comparison of Incompatible vs. Compatible Interactions Using Dual RNASeq.","description":"<i>Leptosphaeria maculans</i> causes blackleg disease, which is one of the most destructive diseases of canola (<i>Brassica napus</i> L.). Due to the erosion of the current resistance in <i>B. napus</i>, it is pivotal to introduce new resistant genotypes to the growers. This study evaluated the potential of <i>Rlm7</i> gene as resistance to its corresponding avirulence <i>AvrLm7</i> gene is abundant. The <i>Rlm7</i> line was inoculated with <i>L. maculans</i> isolate with <i>AvrLm7</i>; UMAvr7; and the CRISPR/Cas9 knockout <i>AvrLm7</i> mutant, umavr7, of the same isolate to cause incompatible and compatible interactions, respectively. Dual RNA-seq showed differential gene expressions in both interactions. High expressions of virulence-related pathogen genes-CAZymes, merops, and effector p","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-06-18T03:09:43.063Z","creation":"2025-04-04T22:13:18.79Z"},"accession":"S-EPMC8999614","cross_references":{"pubmed":["35409323"],"doi":["10.3390/ijms23073964"]}}