biostudies-literatureUnknown23(7)Padmathilake KRE<i>Leptosphaeria maculans</i> causes blackleg disease, which is one of the most destructive diseases of canola (<i>Brassica napus</i> L.). Due to the erosion of the current resistance in <i>B. napus</i>, it is pivotal to introduce new resistant genotypes to the growers. This study evaluated the potential of <i>Rlm7</i> gene as resistance to its corresponding avirulence <i>AvrLm7</i> gene is abundant. The <i>Rlm7</i> line was inoculated with <i>L. maculans</i> isolate with <i>AvrLm7</i>; UMAvr7; and the CRISPR/Cas9 knockout <i>AvrLm7</i> mutant, umavr7, of the same isolate to cause incompatible and compatible interactions, respectively. Dual RNA-seq showed differential gene expressions in both interactions. High expressions of virulence-related pathogen genes-CAZymes, merops, and effector pInternational journal of molecular sciences3964https://www.ebi.ac.uk/biostudies/studies/S-EPMC8999614biostudies-literature<i>Leptosphaeria maculans</i>-<i>Brassica napus</i> Battle: A Comparison of Incompatible vs. Compatible Interactions Using Dual RNASeq.PMC8999614Padmathilake KREFernando WGDfalse<i>Leptosphaeria maculans</i>-<i>Brassica napus</i> Battle: A Comparison of Incompatible vs. Compatible Interactions Using Dual RNASeq.<i>Leptosphaeria maculans</i> causes blackleg disease, which is one of the most destructive diseases of canola (<i>Brassica napus</i> L.). Due to the erosion of the current resistance in <i>B. napus</i>, it is pivotal to introduce new resistant genotypes to the growers. This study evaluated the potential of <i>Rlm7</i> gene as resistance to its corresponding avirulence <i>AvrLm7</i> gene is abundant. The <i>Rlm7</i> line was inoculated with <i>L. maculans</i> isolate with <i>AvrLm7</i>; UMAvr7; and the CRISPR/Cas9 knockout <i>AvrLm7</i> mutant, umavr7, of the same isolate to cause incompatible and compatible interactions, respectively. Dual RNA-seq showed differential gene expressions in both interactions. High expressions of virulence-related pathogen genes-CAZymes, merops, and effector p2022-01-01T00:00:00Z2022 Apr2026-06-18T03:09:43.063Z2025-04-04T22:13:18.79ZS-EPMC89996143540932310.3390/ijms23073964