biostudies-literatureSu ZNational Natural Science Foundation of ChinaSanming Project of Medicine in Shenzhen853529https://www.ebi.ac.uk/biostudies/studies/S-EPMC9001885biostudies-literatureUnknown13<b>Objective:</b> It aimed to construct the miRNA-mRNA regulatory network related to ion channel genes in mesial temporal lobe epilepsy (mTLE), and further identify the vital node in the network. <b>Methods:</b> Firstly, we identified ion channel-related differentially expressed genes (DEGs) in mTLE using the IUPHAR/BPS Guide to Pharmacology (GTP) database, neXtProt database, GeneCards database, and the high-throughput sequencing dataset. Then the STRING online database was used to construct a protein-protein interaction (PPI) network of DEGs, and the hub module in the PPI network was identified using the cytoHubba plug-in of Cytoscape software. In addition, the Single Cell Portal database was used to distinguish genes expression in different cell types. Based on the TarBase database, EpimFrontiers in geneticsIdentification of Ion Channel-Related Genes and miRNA-mRNA Networks in Mesial Temporal Lobe Epilepsy.PMC900188582071447 81571266 81771405Liu XPeng YZhou LLi YSu ZChen SZhao KfalseIdentification of Ion Channel-Related Genes and miRNA-mRNA Networks in Mesial Temporal Lobe Epilepsy.<b>Objective:</b> It aimed to construct the miRNA-mRNA regulatory network related to ion channel genes in mesial temporal lobe epilepsy (mTLE), and further identify the vital node in the network. <b>Methods:</b> Firstly, we identified ion channel-related differentially expressed genes (DEGs) in mTLE using the IUPHAR/BPS Guide to Pharmacology (GTP) database, neXtProt database, GeneCards database, and the high-throughput sequencing dataset. Then the STRING online database was used to construct a protein-protein interaction (PPI) network of DEGs, and the hub module in the PPI network was identified using the cytoHubba plug-in of Cytoscape software. In addition, the Single Cell Portal database was used to distinguish genes expression in different cell types. Based on the TarBase database, Epim2022-01-01T00:00:00Z20222026-06-20T03:19:46.771Z2025-04-06T02:55:00.724ZS-EPMC90018853542284010.3389/fgene.2022.853529