{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chaurasia R"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS"],"pagination":["859680"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9002632"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Leptospirosis is a globally important neglected zoonotic disease. Previous data suggest that a family of virulence-modifying (VM) proteins (PF07598) is a distinctive feature of group I pathogenic <i>Leptospira</i> that evolved as important virulence determinants. Here, we show that one such VM protein, LA3490 (also known as Q8F0K3), is expressed by <i>Leptospira interrogans</i> serovar Lai, as a secreted genotoxin that is potently cytotoxic to human cells. Structural homology searches using Phyre2 suggested that VM proteins are novel R-type lectins containing tandem N-terminal ricin B-chain-like β-trefoil domains. Recombinant LA3490 (rLA3490) and an N-terminal fragment, t3490, containing only the predicted ricin B domain, bound to the terminal galactose and N-acetyl-galactosamine residues,"],"journal":["Frontiers in microbiology"],"pubmed_title":["Pathogenic <i>Leptospira</i> Evolved a Unique Gene Family Comprised of Ricin B-Like Lectin Domain-Containing Cytotoxins."],"pmcid":["PMC9002632"],"funding_grant_id":["R01 AI108276","U19 AI115658","R21 AI115273","R21 AI164106"],"pubmed_authors":["Matthias MA","Marroquin AS","Vinetz JM","Chaurasia R"],"additional_accession":[]},"is_claimable":false,"name":"Pathogenic <i>Leptospira</i> Evolved a Unique Gene Family Comprised of Ricin B-Like Lectin Domain-Containing Cytotoxins.","description":"Leptospirosis is a globally important neglected zoonotic disease. Previous data suggest that a family of virulence-modifying (VM) proteins (PF07598) is a distinctive feature of group I pathogenic <i>Leptospira</i> that evolved as important virulence determinants. Here, we show that one such VM protein, LA3490 (also known as Q8F0K3), is expressed by <i>Leptospira interrogans</i> serovar Lai, as a secreted genotoxin that is potently cytotoxic to human cells. Structural homology searches using Phyre2 suggested that VM proteins are novel R-type lectins containing tandem N-terminal ricin B-chain-like β-trefoil domains. Recombinant LA3490 (rLA3490) and an N-terminal fragment, t3490, containing only the predicted ricin B domain, bound to the terminal galactose and N-acetyl-galactosamine residues,","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-07-14T14:41:55.296Z","creation":"2024-11-20T17:18:51.95Z"},"accession":"S-EPMC9002632","cross_references":{"pubmed":["35422779"],"doi":["10.3389/fmicb.2022.859680"]}}