biostudies-literatureUnknown22(1)Patel N<h4>Background</h4>Female reproductive tract dysbiosis impacts implantation. However, whether gut dysbiosis influences implantation failure and whether it accompanies reproductive tract dysbiosis remains scantly explored. Herein, we examined the gut-vaginal microbiota axis in infertile women.<h4>Methods</h4>We recruited 11 fertile women as the controls, and a cohort of 20 infertile women, 10 of whom had recurrent implantation failure (RIF), and another 10 had unexplained infertility (UE). Using amplicon sequencing, which employs PCR to create sequences of DNA called amplicon, we compared the diversity, structure, and composition of faecal and vaginal bacteria of the controls with that of the infertile cohort. Of note, we could only sequence 8 vaginal samples in each group (n = 24/31).<h4>RBMC women's health113https://www.ebi.ac.uk/biostudies/studies/S-EPMC9004033biostudies-literatureDistinct gut and vaginal microbiota profile in women with recurrent implantation failure and unexplained infertility.PMC9004033Patel NNathani NPandit RParekh BPal SPatel MJoshi CfalseDistinct gut and vaginal microbiota profile in women with recurrent implantation failure and unexplained infertility.<h4>Background</h4>Female reproductive tract dysbiosis impacts implantation. However, whether gut dysbiosis influences implantation failure and whether it accompanies reproductive tract dysbiosis remains scantly explored. Herein, we examined the gut-vaginal microbiota axis in infertile women.<h4>Methods</h4>We recruited 11 fertile women as the controls, and a cohort of 20 infertile women, 10 of whom had recurrent implantation failure (RIF), and another 10 had unexplained infertility (UE). Using amplicon sequencing, which employs PCR to create sequences of DNA called amplicon, we compared the diversity, structure, and composition of faecal and vaginal bacteria of the controls with that of the infertile cohort. Of note, we could only sequence 8 vaginal samples in each group (n = 24/31).<h4>R2022-01-01T00:00:00Z2022 Apr2025-08-12T03:04:47.414Z2025-04-06T12:19:32.541ZS-EPMC90040333541387510.1186/s12905-022-01681-6