{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fahad AS"],"funding":["University of Kansas Cancer Center","University of Minnesota Foundation","NIAID NIH HHS","Randy Shaver Cancer Research and Community Fund","NCI NIH HHS","National Institutes of Health","US Department of Defense","Wellcome Trust","NIH HHS","NIGMS NIH HHS","Richard M. Schulze Family Foundation"],"pagination":["gzab034"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9005053"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35"],"pubmed_abstract":["Functional analyses of the T cell receptor (TCR) landscape can reveal critical information about protection from disease and molecular responses to vaccines. However, it has proven difficult to combine advanced next-generation sequencing technologies with methods to decode the peptide-major histocompatibility complex (pMHC) specificity of individual TCRs. We developed a new high-throughput approach to enable repertoire-scale functional evaluations of natively paired TCRs. In particular, we leveraged the immortalized nature of physically linked TCRα:β amplicon libraries to analyze binding against multiple recombinant pMHCs on a repertoire scale, and to exemplify the utility of this approach, we also performed affinity-based functional mapping in conjunction with quantitative next-generation"],"journal":["Protein engineering, design & selection : PEDS"],"pubmed_title":["Immortalization and functional screening of natively paired human T cell receptor repertoires."],"pmcid":["PMC9005053"],"funding_grant_id":["P20 GM103418","P20 GM103638","100326/Z/12/Z","DP5OD023118","R21 AI166396","W81XWH1810296","R21 CA230487","P20GM103638","P20GM103418","DP5 OD023118","R21CA230487"],"pubmed_authors":["Douek DC","Llewellyn-Lacey S","Price DA","Laflin AD","Fahad AS","DeKosky BJ","Madan B","Balfour HH","Matus-Nicodemos R","Koup RA","Boyle N","Wolfe J","Chung CY","Lopez Acevedo SN","Ladi RR","Gutierrez-Gonzalez MF","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"Immortalization and functional screening of natively paired human T cell receptor repertoires.","description":"Functional analyses of the T cell receptor (TCR) landscape can reveal critical information about protection from disease and molecular responses to vaccines. However, it has proven difficult to combine advanced next-generation sequencing technologies with methods to decode the peptide-major histocompatibility complex (pMHC) specificity of individual TCRs. We developed a new high-throughput approach to enable repertoire-scale functional evaluations of natively paired TCRs. In particular, we leveraged the immortalized nature of physically linked TCRα:β amplicon libraries to analyze binding against multiple recombinant pMHCs on a repertoire scale, and to exemplify the utility of this approach, we also performed affinity-based functional mapping in conjunction with quantitative next-generation","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-06-03T17:08:08.021Z","creation":"2025-04-21T15:56:58.839Z"},"accession":"S-EPMC9005053","cross_references":{"pubmed":["35174859"],"doi":["10.1093/protein/gzab034"]}}