{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Meng Q"],"pubmed_abstract":["Spinach is a typical light-sensitive plant. Long days can induce early bolting, thereby influencing the regional adaptation, quality, and vegetative yield of spinach. However, the genes and genetic mechanisms underlying this trait in spinach remain unclear. In this study, a major quantitative trait locus (QTL) <i>q</i>BT1.1, was mapped on chromosome 1 using a BC<sub>1</sub> population (BC<sub>1a</sub>) derived from 12S3 (late-bolting recurrent lines) and 12S4 (early bolting lines) with specific-locus amplified fragment (SLAF) markers and Kompetitive Allele Specific PCR (KASP) markers. The <i>q</i>BT1.1 locus was further confirmed and narrowed down to 0.56 Mb by using a large BC<sub>1</sub> (BC<sub>1b</sub>) population and an F<sub>2</sub> population using the above KASP markers and the other 20 KASP markers. Within this region, two putative genes, namely, <i>SpFLC</i> and <i>SpCOL</i>14, were of interest due to their relationship with flower regulatory pathways. For <i>SpCOL</i>14, we found multiple variations in the promoter, and the expression pattern was consistent with bolting stages. <i>SpCOL</i>14 was therefore assumed to the best candidate gene for bolting. Overall, our results provide a basis for understanding the molecular mechanisms of bolting in spinach and contribute to the breeding of diverse spinach germplasms for adaptation to different regions."],"journal":["Frontiers in plant science"],"pagination":["850810"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9006512"],"repository":["biostudies-literature"],"pubmed_title":["Quantitative Trait Locus Mapping and Identification of Candidate Genes Controlling Bolting in Spinach (<i>Spinacia oleracea</i> L.)."],"pmcid":["PMC9006512"],"pubmed_authors":["Wu J","Qian W","Liu Z","Meng Q","Zhang H","She H","Wang X","Feng C","Xu Z"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative Trait Locus Mapping and Identification of Candidate Genes Controlling Bolting in Spinach (<i>Spinacia oleracea</i> L.).","description":"Spinach is a typical light-sensitive plant. Long days can induce early bolting, thereby influencing the regional adaptation, quality, and vegetative yield of spinach. However, the genes and genetic mechanisms underlying this trait in spinach remain unclear. In this study, a major quantitative trait locus (QTL) <i>q</i>BT1.1, was mapped on chromosome 1 using a BC<sub>1</sub> population (BC<sub>1a</sub>) derived from 12S3 (late-bolting recurrent lines) and 12S4 (early bolting lines) with specific-locus amplified fragment (SLAF) markers and Kompetitive Allele Specific PCR (KASP) markers. The <i>q</i>BT1.1 locus was further confirmed and narrowed down to 0.56 Mb by using a large BC<sub>1</sub> (BC<sub>1b</sub>) population and an F<sub>2</sub> population using the above KASP markers and the other 20 KASP markers. Within this region, two putative genes, namely, <i>SpFLC</i> and <i>SpCOL</i>14, were of interest due to their relationship with flower regulatory pathways. For <i>SpCOL</i>14, we found multiple variations in the promoter, and the expression pattern was consistent with bolting stages. <i>SpCOL</i>14 was therefore assumed to the best candidate gene for bolting. Overall, our results provide a basis for understanding the molecular mechanisms of bolting in spinach and contribute to the breeding of diverse spinach germplasms for adaptation to different regions.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2024-10-18T05:43:16.662Z","creation":"2024-10-18T05:43:16.662Z"},"accession":"S-EPMC9006512","cross_references":{"pubmed":["35432424"],"doi":["10.3389/fpls.2022.850810"]}}