biostudies-literatureUnknown13Hwang IS<i>Clavibacter michiganensis</i>, a Gram-positive plant-pathogenic bacterium, utilizes apoplastic effectors for disease development in host plants. Here, we determine the roles of Pat-1_Cm (a putative serine protease) in pathogenicity and plant immunity. Pat-1_Cm was found to be a genuine secreted protein, and the secreted mature form did not carry the first 33 amino acids predicted to be a signal peptide (SP). The <i>pat-1_Cm</i> mutant impaired to cause wilting, but still caused canker symptom in tomato. Moreover, this mutant failed to trigger the hypersensitive response (HR) in a nonhost <i>Nicotiana tabacum</i>. Among orthologs and paralogs of <i>pat-1_Cm</i> , only <i>chp-7_Cs</i> from <i>Clavibacter sepedonicus</i>, a potato pathogen,Frontiers in plant science888290https://www.ebi.ac.uk/biostudies/studies/S-EPMC9006514biostudies-literatureAn Apoplastic Effector Pat-1_Cm of the Gram-Positive Bacterium <i>Clavibacter michiganensis</i> Acts as Both a Pathogenicity Factor and an Immunity Elicitor in Plants.PMC9006514Sohn KHChoi DHwang ISPark IWOh EJSong ELee YOh CSfalseAn Apoplastic Effector Pat-1_Cm of the Gram-Positive Bacterium <i>Clavibacter michiganensis</i> Acts as Both a Pathogenicity Factor and an Immunity Elicitor in Plants.<i>Clavibacter michiganensis</i>, a Gram-positive plant-pathogenic bacterium, utilizes apoplastic effectors for disease development in host plants. Here, we determine the roles of Pat-1_Cm (a putative serine protease) in pathogenicity and plant immunity. Pat-1_Cm was found to be a genuine secreted protein, and the secreted mature form did not carry the first 33 amino acids predicted to be a signal peptide (SP). The <i>pat-1_Cm</i> mutant impaired to cause wilting, but still caused canker symptom in tomato. Moreover, this mutant failed to trigger the hypersensitive response (HR) in a nonhost <i>Nicotiana tabacum</i>. Among orthologs and paralogs of <i>pat-1_Cm</i> , only <i>chp-7_Cs</i> from <i>Clavibacter sepedonicus</i>, a potato pathogen,2022-01-01T00:00:00Z20222026-05-31T07:00:08.966Z2025-02-19T01:55:29.234ZS-EPMC90065143543242710.3389/fpls.2022.888290