biostudies-literatureYarishkin ONEI NIH HHSNCATS NIH HHSU.S. Army Medical Research Acquisition ActivityNational Institutes of Health805076https://www.ebi.ac.uk/biostudies/studies/S-EPMC9008486biostudies-literatureUnknown13Trabecular meshwork (TM) cells are phagocytic cells that employ mechanotransduction to actively regulate intraocular pressure. Similar to macrophages, they express scavenger receptors and participate in antigen presentation within the immunosuppressive milieu of the anterior eye. Changes in pressure deform and compress the TM, altering their control of aqueous humor outflow but it is not known whether transducer activation shapes temporal signaling. The present study combines electrophysiology, histochemistry and functional imaging with gene silencing and heterologous expression to gain insight into Ca²⁺ signaling downstream from TRPV4 (Transient Receptor Potential Vanilloid 4), a stretch-activated polymodal cation channel. Human TM cells respond to the TRPV4 agonist GSK1016790AFrontiers in immunologyEmergent Temporal Signaling in Human Trabecular Meshwork Cells: Role of TRPV4-TRPM4 Interactions.PMC9008486R01 EY027920R01EY027920, R01EY031817, P30EY014800, T32EY024234P30 EY014800VR200079R01 EY031817UL1 TR002538T32 EY024234Redmon SNHwang EMRudzitis CNFreichel MOverby DVazquez-Chona FBaumann JMBertrand JYarishkin OPhuong TTTKrizaj Dvan Battenburg-Sherwood JLakk MfalseEmergent Temporal Signaling in Human Trabecular Meshwork Cells: Role of TRPV4-TRPM4 Interactions.Trabecular meshwork (TM) cells are phagocytic cells that employ mechanotransduction to actively regulate intraocular pressure. Similar to macrophages, they express scavenger receptors and participate in antigen presentation within the immunosuppressive milieu of the anterior eye. Changes in pressure deform and compress the TM, altering their control of aqueous humor outflow but it is not known whether transducer activation shapes temporal signaling. The present study combines electrophysiology, histochemistry and functional imaging with gene silencing and heterologous expression to gain insight into Ca²⁺ signaling downstream from TRPV4 (Transient Receptor Potential Vanilloid 4), a stretch-activated polymodal cation channel. Human TM cells respond to the TRPV4 agonist GSK1016790A2022-01-01T00:00:00Z20222026-06-19T03:10:53.69Z2025-02-19T00:56:46.114ZS-EPMC90084863543230210.3389/fimmu.2022.805076