{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McIlvin MR"],"funding":["National Institute of Health","G.B Moore Foundation","NIGMS NIH HHS","National Science Foundation"],"pagination":["4589-4597"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9009399"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(9)"],"pubmed_abstract":["Metaproteomics is a powerful analytical approach that can assess the functional capabilities deployed by microbial communities in both environmental and biomedical microbiome settings. Yet, the mass spectra resulting from these mixed biological communities are challenging to obtain due to the high number of low intensity peak features. The use of multiple dimensions of chromatographic separation prior to mass spectrometry analyses has been applied to proteomics previously but can require increased sampling handling and instrument time. Here, we demonstrate an automated online comprehensive active modulation two-dimensional liquid chromatography method for metaproteome sample analysis. A high pH PLRP-S column was used in the first dimension followed by low pH separation in the second dimens"],"journal":["Journal of proteome research"],"pubmed_title":["Online Nanoflow Two-Dimension Comprehensive Active Modulation Reversed Phase-Reversed Phase Liquid Chromatography High-Resolution Mass Spectrometry for Metaproteomics of Environmental and Microbiome Samples."],"pmcid":["PMC9009399"],"funding_grant_id":["R01 GM135709","R01GM135709","1925445, 1850719"],"pubmed_authors":["McIlvin MR","Saito MA"],"additional_accession":[]},"is_claimable":false,"name":"Online Nanoflow Two-Dimension Comprehensive Active Modulation Reversed Phase-Reversed Phase Liquid Chromatography High-Resolution Mass Spectrometry for Metaproteomics of Environmental and Microbiome Samples.","description":"Metaproteomics is a powerful analytical approach that can assess the functional capabilities deployed by microbial communities in both environmental and biomedical microbiome settings. Yet, the mass spectra resulting from these mixed biological communities are challenging to obtain due to the high number of low intensity peak features. The use of multiple dimensions of chromatographic separation prior to mass spectrometry analyses has been applied to proteomics previously but can require increased sampling handling and instrument time. Here, we demonstrate an automated online comprehensive active modulation two-dimensional liquid chromatography method for metaproteome sample analysis. A high pH PLRP-S column was used in the first dimension followed by low pH separation in the second dimens","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-04T13:30:33.787Z","creation":"2025-02-19T01:07:10.883Z"},"accession":"S-EPMC9009399","cross_references":{"pubmed":["34384028"],"doi":["10.1021/acs.jproteome.1c00588"]}}