biostudies-literatureLi MNatural Science Foundation of Zhejiang ProvinceNational Natural Science Foundation of China6349https://www.ebi.ac.uk/biostudies/studies/S-EPMC9012805biostudies-literatureUnknown12(1)The development of 3D printing has recently attracted significant attention on constructing complex three-dimensional physiological microenvironments. However, it is very challenging to provide a bio-ink with cell-harmless and high mold accuracy during extrusion in 3D printing. To overcome this issue, a technique improving the shear-thinning performance of semi-IPN bio-ink, which is universally applicable to all alginate/gelatin-based materials, was developed. Semi-IPN bio-ink prepared by cyclic heating-cooling treatment in this study can reduce the cell damage without sacrificing the accuracy of the scaffolds for its excellent shear-thinning performance. A more than 15% increase in post-printing Cell viability verified the feasibility of the strategy. Moreover, the bio-ink with low molecuScientific reportsHigh-cytocompatible semi-IPN bio-ink with wide molecular weight distribution for extrusion 3D bioprinting.PMC9012805No. 61873078LY21H170002Liu KYao DShi TWang HYue XLi MfalseHigh-cytocompatible semi-IPN bio-ink with wide molecular weight distribution for extrusion 3D bioprinting.The development of 3D printing has recently attracted significant attention on constructing complex three-dimensional physiological microenvironments. However, it is very challenging to provide a bio-ink with cell-harmless and high mold accuracy during extrusion in 3D printing. To overcome this issue, a technique improving the shear-thinning performance of semi-IPN bio-ink, which is universally applicable to all alginate/gelatin-based materials, was developed. Semi-IPN bio-ink prepared by cyclic heating-cooling treatment in this study can reduce the cell damage without sacrificing the accuracy of the scaffolds for its excellent shear-thinning performance. A more than 15% increase in post-printing Cell viability verified the feasibility of the strategy. Moreover, the bio-ink with low molecu2022-01-01T00:00:00Z2022 Apr2025-04-04T20:37:17.081Z2025-02-19T03:41:56.931ZS-EPMC90128053542880010.1038/s41598-022-10338-1