biostudies-literatureCipollari SNational Center for Advancing Translational SciencesNCATS NIH HHSNCI NIH HHSProstate Cancer Foundation521-529https://www.ebi.ac.uk/biostudies/studies/S-EPMC9014804biostudies-literatureUnknown81(9)<h4>Background</h4>Tissue clearing technologies have enabled remarkable advancements for in situ characterization of tissues and exploration of the three-dimensional (3D) relationships between cells, however, these studies have predominantly been performed in non-human tissues and correlative assessment with clinical imaging has yet to be explored. We sought to evaluate the feasibility of tissue clearing technologies for 3D imaging of intact human prostate and the mapping of structurally and molecularly preserved pathology data with multi-parametric volumetric MR imaging (mpMRI).<h4>Methods</h4>Whole-mount prostates were processed with either hydrogel-based CLARITY or solvent-based iDISCO. The samples were stained with a nuclear dye or fluorescently labeled with antibodies against androgenThe ProstateTissue clearing techniques for three-dimensional optical imaging of intact human prostate and correlations with multi-parametric MRI.PMC9014804KL2TR001882KL2 TR001882Stewart Rahr–PCF Young Investigator AwardP50 CA092131Sung KCipollari SHuang DJamshidi NHuang JDu LMargolis DJKuo MDReiter REfalseTissue clearing techniques for three-dimensional optical imaging of intact human prostate and correlations with multi-parametric MRI.<h4>Background</h4>Tissue clearing technologies have enabled remarkable advancements for in situ characterization of tissues and exploration of the three-dimensional (3D) relationships between cells, however, these studies have predominantly been performed in non-human tissues and correlative assessment with clinical imaging has yet to be explored. We sought to evaluate the feasibility of tissue clearing technologies for 3D imaging of intact human prostate and the mapping of structurally and molecularly preserved pathology data with multi-parametric volumetric MR imaging (mpMRI).<h4>Methods</h4>Whole-mount prostates were processed with either hydrogel-based CLARITY or solvent-based iDISCO. The samples were stained with a nuclear dye or fluorescently labeled with antibodies against androgen2021-01-01T00:00:00Z2021 Jun2025-04-04T20:36:49.379Z2025-02-18T23:36:22.637ZS-EPMC90148043387683810.1002/pros.24129