{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["1(1)"],"submitter":["Bertan F"],"funding":["Innovative Medicines Initiative","DFG","Horizon 2020 Marie Skłodowska-Curie Actions"],"pubmed_abstract":["Mitochondria sustain the energy demand of the cell. The composition and functional state of the mitochondrial oxidative phosphorylation system are informative indicators of organelle bioenergetic capacity. Here, we describe a highly sensitive and reproducible method for a single-cell quantification of mitochondrial CI- and CIV-containing respiratory supercomplexes (CI∗CIV-SCs) as an alternative means of assessing mitochondrial respiratory chain integrity. We apply a proximity ligation assay (PLA) and stain CI∗CIV-SCs in fixed human and mouse brains, tumorigenic cells, induced pluripotent stem cells (iPSCs) and iPSC-derived neural precursor cells (NPCs), and neurons. Spatial visualization of CI∗CIV-SCs enables the detection of mitochondrial lesions in various experimental models, including "],"journal":["Cell reports methods"],"pagination":["100002"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9017192"],"repository":["biostudies-literature"],"pubmed_title":["Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution."],"pmcid":["PMC9017192"],"pubmed_authors":["Bertan F","Marsal-Cots A","Nicotera P","Scifo E","Prehn JHM","Peitz M","Bano D","Ehninger D","Guranda M","Piazzesi A","Wischhof L","Stork M","Jackson J"],"additional_accession":[]},"is_claimable":false,"name":"Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution.","description":"Mitochondria sustain the energy demand of the cell. The composition and functional state of the mitochondrial oxidative phosphorylation system are informative indicators of organelle bioenergetic capacity. Here, we describe a highly sensitive and reproducible method for a single-cell quantification of mitochondrial CI- and CIV-containing respiratory supercomplexes (CI∗CIV-SCs) as an alternative means of assessing mitochondrial respiratory chain integrity. We apply a proximity ligation assay (PLA) and stain CI∗CIV-SCs in fixed human and mouse brains, tumorigenic cells, induced pluripotent stem cells (iPSCs) and iPSC-derived neural precursor cells (NPCs), and neurons. Spatial visualization of CI∗CIV-SCs enables the detection of mitochondrial lesions in various experimental models, including ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-09T21:52:01.982Z","creation":"2024-11-20T22:37:38.162Z"},"accession":"S-EPMC9017192","cross_references":{"pubmed":["35474694"],"doi":["10.1016/j.crmeth.2021.100002"]}}