{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lawson MR"],"funding":["A.P. Giannini Foundation","Cystic Fibrosis Foundation","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["876-882"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9017434"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["373(6557)"],"pubmed_abstract":["Translation termination, which liberates a nascent polypeptide from the ribosome specifically at stop codons, must occur accurately and rapidly. We established single-molecule fluorescence assays to track the dynamics of ribosomes and two requisite release factors (eRF1 and eRF3) throughout termination using an in vitro-reconstituted yeast translation system. We found that the two eukaryotic release factors bound together to recognize stop codons rapidly and elicit termination through a tightly regulated, multistep process that resembles transfer RNA selection during translation elongation. Because the release factors are conserved from yeast to humans, the molecular events that underlie yeast translation termination are likely broadly fundamental to eukaryotic protein synthesis."],"journal":["Science (New York, N.Y.)"],"pubmed_title":["Mechanisms that ensure speed and fidelity in eukaryotic translation termination."],"pmcid":["PMC9017434"],"funding_grant_id":["R01 GM113078","R37GM059425","PUGLISI20GO","R01 GM051266","R01GM51266","R01GM113078","R37 GM059425"],"pubmed_authors":["Puglisi JD","Lawson MR","Lessen LN","Green R","Corsepius NC","Prabhakar A","Wang J"],"additional_accession":[]},"is_claimable":false,"name":"Mechanisms that ensure speed and fidelity in eukaryotic translation termination.","description":"Translation termination, which liberates a nascent polypeptide from the ribosome specifically at stop codons, must occur accurately and rapidly. We established single-molecule fluorescence assays to track the dynamics of ribosomes and two requisite release factors (eRF1 and eRF3) throughout termination using an in vitro-reconstituted yeast translation system. We found that the two eukaryotic release factors bound together to recognize stop codons rapidly and elicit termination through a tightly regulated, multistep process that resembles transfer RNA selection during translation elongation. Because the release factors are conserved from yeast to humans, the molecular events that underlie yeast translation termination are likely broadly fundamental to eukaryotic protein synthesis.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Aug","modification":"2026-05-09T23:45:09.737Z","creation":"2025-04-04T22:14:56.19Z"},"accession":"S-EPMC9017434","cross_references":{"pubmed":["34413231"],"doi":["10.1126/science.abi7801"]}}