biostudies-literatureSong JNational Natural Science Foundation of Chinae614https://www.ebi.ac.uk/biostudies/studies/S-EPMC9017637biostudies-literatureUnknown10(5)<h4>Introduction</h4>This study aimed to explore the association between the IRAK4 polymorphism rs4251545 and the severity of enterovirus 71 (EV71) infection in Chinese children.<h4>Methods</h4>We analyzed the IRAK4 polymorphism rs4251545 in 617 EV71-infected patients and 410 controls using the improved multiplex ligation detection reaction. IRAK4 mRNA expression was tested by qRT-PCR. Serum concentrations of IL-6 and NF-κB were detected using ELISA.<h4>Results</h4>The frequencies of the GA + AA genotype and A allele in the mild EV71 infection group and in the severe EV71 infection group were significantly higher than those in the normal control group. The frequency of the GA + AA genotype and A allele in severely infected EV71 patients was markedly higher than that in mildly infected EV71Immunity, inflammation and diseaseAssociation of the IRAK4 rs4251545 genetic polymorphism with severity of enterovirus-71 infection in Chinese children.PMC901763781871246Pan XLiu YFan FGuo YChen ZYi LLi FLiu PYang CSong JZhao HfalseAssociation of the IRAK4 rs4251545 genetic polymorphism with severity of enterovirus-71 infection in Chinese children.<h4>Introduction</h4>This study aimed to explore the association between the IRAK4 polymorphism rs4251545 and the severity of enterovirus 71 (EV71) infection in Chinese children.<h4>Methods</h4>We analyzed the IRAK4 polymorphism rs4251545 in 617 EV71-infected patients and 410 controls using the improved multiplex ligation detection reaction. IRAK4 mRNA expression was tested by qRT-PCR. Serum concentrations of IL-6 and NF-κB were detected using ELISA.<h4>Results</h4>The frequencies of the GA + AA genotype and A allele in the mild EV71 infection group and in the severe EV71 infection group were significantly higher than those in the normal control group. The frequency of the GA + AA genotype and A allele in severely infected EV71 patients was markedly higher than that in mildly infected EV712022-01-01T00:00:00Z2022 May2025-04-20T00:18:04.912Z2025-02-19T01:53:53.583ZS-EPMC90176373547843910.1002/iid3.614