{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rather HA"],"funding":["Center for Research on Substance Use and Addiction, Wake Forest School of Medicine and NIH"],"pagination":["510"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9026784"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(4)"],"pubmed_abstract":["Cocaine use disorder has been reported to cause transgenerational effects. However, due to the lack of standardized biomarkers, the effects of cocaine use during pregnancy on postnatal development and long-term neurobiological and behavioral outcomes have not been investigated thoroughly. Therefore, in this study, we examined extracellular vesicles (EVs) in adult (~12 years old) female and male rhesus monkeys prenatally exposed to cocaine (n = 11) and controls (n = 9). EVs were isolated from the cerebrospinal fluid (CSF) and characterized for the surface expression of specific tetraspanins, concentration (particles/mL), size distribution, and cargo proteins by mass spectrometry (MS). Transmission electron microscopy following immunogold labeling for tetraspanins (CD63, CD9, and CD81) confi"],"journal":["Biomolecules"],"pubmed_title":["Mass Spectrometry-Based Proteome Profiling of Extracellular Vesicles Derived from the Cerebrospinal Fluid of Adult Rhesus Monkeys Exposed to Cocaine throughout Gestation."],"pmcid":["PMC9026784"],"funding_grant_id":["R01 DA049267 and R01 DA025120"],"pubmed_authors":["Misra BB","Rather HA","Mishra S","Lee J","Hamilton LR","Gould RW","Nader MA","Nader SH","Deep G","Su Y","Singh S","Kumar A","Furdui CM"],"additional_accession":[]},"is_claimable":false,"name":"Mass Spectrometry-Based Proteome Profiling of Extracellular Vesicles Derived from the Cerebrospinal Fluid of Adult Rhesus Monkeys Exposed to Cocaine throughout Gestation.","description":"Cocaine use disorder has been reported to cause transgenerational effects. However, due to the lack of standardized biomarkers, the effects of cocaine use during pregnancy on postnatal development and long-term neurobiological and behavioral outcomes have not been investigated thoroughly. Therefore, in this study, we examined extracellular vesicles (EVs) in adult (~12 years old) female and male rhesus monkeys prenatally exposed to cocaine (n = 11) and controls (n = 9). EVs were isolated from the cerebrospinal fluid (CSF) and characterized for the surface expression of specific tetraspanins, concentration (particles/mL), size distribution, and cargo proteins by mass spectrometry (MS). Transmission electron microscopy following immunogold labeling for tetraspanins (CD63, CD9, and CD81) confi","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-22T19:14:18.536Z","creation":"2025-04-06T02:39:31.48Z"},"accession":"S-EPMC9026784","cross_references":{"pubmed":["35454099"],"doi":["10.3390/biom12040510"]}}