biostudies-literatureMason MRJVan den Houtenfonds; the International Spinal Research TrustThe Francis Crick InstituteDutch Research Council (NWO)ZonMwInternational Foundation for Research in Paraplegia1242-1262https://www.ebi.ac.uk/biostudies/studies/S-EPMC9029231biostudies-literatureUnknown31(8)The regeneration-associated gene (RAG) expression program is activated in injured peripheral neurons after axotomy and enables long-distance axon re-growth. Over 1000 genes are regulated, and many transcription factors are upregulated or activated as part of this response. However, a detailed picture of how RAG expression is regulated is lacking. In particular, the transcriptional targets and specific functions of the various transcription factors are unclear. Jun was the first-regeneration-associated transcription factor identified and the first shown to be functionally important. Here we fully define the role of Jun in the RAG expression program in regenerating facial motor neurons. At 1, 4 and 14 days after axotomy, Jun upregulates 11, 23 and 44% of the RAG program, respectively. Jun fuHuman molecular geneticsThe Jun-dependent axon regeneration gene program: Jun promotes regeneration over plasticity.PMC902923191211043STR 11110039IRP-NL-011/05Behrens AWolzak KRaivich GMason MRJVerhaagen Jvan Erp SfalseThe Jun-dependent axon regeneration gene program: Jun promotes regeneration over plasticity.The regeneration-associated gene (RAG) expression program is activated in injured peripheral neurons after axotomy and enables long-distance axon re-growth. Over 1000 genes are regulated, and many transcription factors are upregulated or activated as part of this response. However, a detailed picture of how RAG expression is regulated is lacking. In particular, the transcriptional targets and specific functions of the various transcription factors are unclear. Jun was the first-regeneration-associated transcription factor identified and the first shown to be functionally important. Here we fully define the role of Jun in the RAG expression program in regenerating facial motor neurons. At 1, 4 and 14 days after axotomy, Jun upregulates 11, 23 and 44% of the RAG program, respectively. Jun fu2022-01-01T00:00:00Z2022 Apr2026-07-14T17:08:55.934Z2025-04-04T07:46:07.004ZS-EPMC90292313471857210.1093/hmg/ddab315