{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He W"],"funding":["Texas A&M AgriLife Research","Texas A&M AgriLife Research"],"pagination":["skac053"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9030142"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["100(4)"],"pubmed_abstract":["This study determined the oxidation of amino acids, glucose and fatty acid in enterocytes of developing chickens. Jejunal enterocytes were isolated from 0-, 7-, 21-, and 42-d-old broiler chickens, and incubated at 40°C for 30 min in Krebs-Henseleit bicarbonate buffer (pH 7.4) containing 5 mM D-glucose and one of the following: 0.5-5 mM L-[U-14C]glutamate, 0.5-5 mM L-[U-14C]glutamine, 0.5-5 mM L-[U-14C]aspartate, 0.5-5 mM L-[U-14C]alanine, 0.5-2 mM [U-14C]palmitate, D-[U-14C]glucose, 0.5-5 mM [U-14C]propionate, and 0.5-5 mM [1-14C]butyrate. 14CO2 produced from each 14C-labeled substrate was collected for determination of radioactivity. Among all the substrates studied, glutamate had the greatest rate of oxidation in enterocytes from 0- to 42-d-old chickens. Glutamate transaminases, rather t"],"journal":["Journal of animal science"],"pubmed_title":["Oxidation of amino acids, glucose, and fatty acids as metabolic fuels in enterocytes of post-hatching developing chickens."],"pmcid":["PMC9030142"],"funding_grant_id":["H-8200"],"pubmed_authors":["Furukawa K","He W","Wu G","Bailey CA"],"additional_accession":[]},"is_claimable":false,"name":"Oxidation of amino acids, glucose, and fatty acids as metabolic fuels in enterocytes of post-hatching developing chickens.","description":"This study determined the oxidation of amino acids, glucose and fatty acid in enterocytes of developing chickens. Jejunal enterocytes were isolated from 0-, 7-, 21-, and 42-d-old broiler chickens, and incubated at 40°C for 30 min in Krebs-Henseleit bicarbonate buffer (pH 7.4) containing 5 mM D-glucose and one of the following: 0.5-5 mM L-[U-14C]glutamate, 0.5-5 mM L-[U-14C]glutamine, 0.5-5 mM L-[U-14C]aspartate, 0.5-5 mM L-[U-14C]alanine, 0.5-2 mM [U-14C]palmitate, D-[U-14C]glucose, 0.5-5 mM [U-14C]propionate, and 0.5-5 mM [1-14C]butyrate. 14CO2 produced from each 14C-labeled substrate was collected for determination of radioactivity. Among all the substrates studied, glutamate had the greatest rate of oxidation in enterocytes from 0- to 42-d-old chickens. Glutamate transaminases, rather t","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-26T14:52:34.21Z","creation":"2025-04-06T14:46:09.313Z"},"accession":"S-EPMC9030142","cross_references":{"pubmed":["35199826"],"doi":["10.1093/jas/skac053"]}}