biostudies-literatureUnknown10(4)Cabrera-Wrooman AElectrolyzed acid solutions produced by different methods have antiseptic properties due to the presence of chlorine and reactive oxygen species. Our aim was to determine whether a controlled-flow electrolyzed acid solution (CFEAS) has the ability to improve wound healing due to its antiseptic and antibiofilm properties. First, we demonstrated in vitro that Gram-negative and Gram-positive bacteria were susceptible to CFEAS, and the effect was partially sustained for 24 h, evidencing antibiofilm activity (<i>p</i> < 0.05, CFEAS-treated vs. controls). The partial cytotoxicity of CFEAS was mainly observed in macrophages after 6 h of treatment; meanwhile, fibroblasts resisted short-lived free radicals (<i>p</i> < 0.05, CFEAS treated vs. controls), perhaps through redox-regulating mechaniMicroorganisms745https://www.ebi.ac.uk/biostudies/studies/S-EPMC9032035biostudies-literatureAntiseptic Effects and Biosafety of a Controlled-Flow Electrolyzed Acid Solution Involve Electrochemical Properties, Rather than Free Radical Presence.PMC9032035Salgado RMSandoval-Cuevas BCabrera-Wrooman AOrtega-Pena SKrotzsch EfalseAntiseptic Effects and Biosafety of a Controlled-Flow Electrolyzed Acid Solution Involve Electrochemical Properties, Rather than Free Radical Presence.Electrolyzed acid solutions produced by different methods have antiseptic properties due to the presence of chlorine and reactive oxygen species. Our aim was to determine whether a controlled-flow electrolyzed acid solution (CFEAS) has the ability to improve wound healing due to its antiseptic and antibiofilm properties. First, we demonstrated in vitro that Gram-negative and Gram-positive bacteria were susceptible to CFEAS, and the effect was partially sustained for 24 h, evidencing antibiofilm activity (<i>p</i> < 0.05, CFEAS-treated vs. controls). The partial cytotoxicity of CFEAS was mainly observed in macrophages after 6 h of treatment; meanwhile, fibroblasts resisted short-lived free radicals (<i>p</i> < 0.05, CFEAS treated vs. controls), perhaps through redox-regulating mechani2022-01-01T00:00:00Z2022 Mar2025-06-01T02:56:46.743Z2025-06-01T02:56:46.743ZS-EPMC90320353545679510.3390/microorganisms10040745