{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Burton TCJ"],"funding":["NCCIH NIH HHS","NHLBI NIH HHS","NIGMS NIH HHS"],"pagination":["110-116"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9032482"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["45"],"pubmed_abstract":["A new isoflavonoid, xanthocerin J, along with previously described xanthocerin A, were isolated from a methanol extract of aerial parts of a traditional American Indian herb, <i>Amorpha canescens</i> Pursh (Asteraceae). The structures of these compounds were characterized using mass spectrometry and NMR based on an isolation protocol using magnetic microbead affinity selection screening (MagMASS) for ligands to the estrogen receptor alpha (ERα). These compounds bound to ERα from an active fraction that exhibited dose-dependent antiestrogenic activity in the <i>in vitro</i> Ishikawa assay. However, these compounds did not exhibit antiestrogenic activity in the cell-based Ishikawa assay. Xanthocerin A and J may exhibit synergistic or additive activity with other compounds found in <i>A. cane"],"journal":["Phytochemistry letters"],"pubmed_title":["Isolation and elucidation of two isoflavonoids from an American Indian plant, <i>Amorpha canescens</i> Pursh, using Magnetic Microbead Affinity Selection Screening (MagMASS) for estrogen receptor alpha ligands."],"pmcid":["PMC9032482"],"funding_grant_id":["P50 AT000155","P41 GM068944","T32 HL134634"],"pubmed_authors":["van Breemen RB","Nikolic D","Ju J","Dietz BM","Soejarto DD","Burton TCJ","Lankin DC","Guo B","Che CT"],"additional_accession":[]},"is_claimable":false,"name":"Isolation and elucidation of two isoflavonoids from an American Indian plant, <i>Amorpha canescens</i> Pursh, using Magnetic Microbead Affinity Selection Screening (MagMASS) for estrogen receptor alpha ligands.","description":"A new isoflavonoid, xanthocerin J, along with previously described xanthocerin A, were isolated from a methanol extract of aerial parts of a traditional American Indian herb, <i>Amorpha canescens</i> Pursh (Asteraceae). The structures of these compounds were characterized using mass spectrometry and NMR based on an isolation protocol using magnetic microbead affinity selection screening (MagMASS) for ligands to the estrogen receptor alpha (ERα). These compounds bound to ERα from an active fraction that exhibited dose-dependent antiestrogenic activity in the <i>in vitro</i> Ishikawa assay. However, these compounds did not exhibit antiestrogenic activity in the cell-based Ishikawa assay. Xanthocerin A and J may exhibit synergistic or additive activity with other compounds found in <i>A. cane","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2025-04-05T14:59:04.19Z","creation":"2025-02-19T02:24:34.79Z"},"accession":"S-EPMC9032482","cross_references":{"pubmed":["35465454"],"doi":["10.1016/j.phytol.2021.07.018"]}}