{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Z"],"funding":["National Natural Science Foundation of China","Natural Science Foundation of Hainan Province","Key Research and Development Project of Hainan Province"],"pagination":["21760-21766"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9034093"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(35)"],"pubmed_abstract":["Ochratoxin A (OTA) is a common food contaminant with multiple toxicities and thus rapid and accurate detection of OTA is indispensable to minimize the threat of OTA to public health. Herein a novel enzyme cascade-amplified immunoassay (ECAIA) based on the mutated nanobody-alkaline phosphatase fusion (mNb-AP) and MnO<sub>2</sub> nanosheets was established for detecting OTA in coffee. The detection principle is that the dual functional mNb-AP could specifically recognize OTA and dephosphorylate the ascorbic acid-2-phosphate (AAP) into ascorbic acid (AA), and the MnO<sub>2</sub> nanosheets mimicking the oxidase could be reduced by AA into Mn<sup>2+</sup> and catalyze the 3,3',5,5'-tetramethyl benzidine into blue oxidized product for quantification. Using the optimal conditions, the ECAIA coul"],"journal":["RSC advances"],"pubmed_title":["Enzyme cascade-amplified immunoassay based on the nanobody-alkaline phosphatase fusion and MnO<sub>2</sub> nanosheets for the detection of ochratoxin A in coffee."],"pmcid":["PMC9034093"],"funding_grant_id":["31901800","320RC509","31760493","2019RC119","ZDYF2020157"],"pubmed_authors":["Liu X","Chen Q","Su B","Cao H","Zhang Z","He Z","Xu H","Zhou Y","Sun Z"],"additional_accession":[]},"is_claimable":false,"name":"Enzyme cascade-amplified immunoassay based on the nanobody-alkaline phosphatase fusion and MnO<sub>2</sub> nanosheets for the detection of ochratoxin A in coffee.","description":"Ochratoxin A (OTA) is a common food contaminant with multiple toxicities and thus rapid and accurate detection of OTA is indispensable to minimize the threat of OTA to public health. Herein a novel enzyme cascade-amplified immunoassay (ECAIA) based on the mutated nanobody-alkaline phosphatase fusion (mNb-AP) and MnO<sub>2</sub> nanosheets was established for detecting OTA in coffee. The detection principle is that the dual functional mNb-AP could specifically recognize OTA and dephosphorylate the ascorbic acid-2-phosphate (AAP) into ascorbic acid (AA), and the MnO<sub>2</sub> nanosheets mimicking the oxidase could be reduced by AA into Mn<sup>2+</sup> and catalyze the 3,3',5,5'-tetramethyl benzidine into blue oxidized product for quantification. Using the optimal conditions, the ECAIA coul","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2025-04-22T01:39:56.193Z","creation":"2025-04-05T20:06:19.856Z"},"accession":"S-EPMC9034093","cross_references":{"pubmed":["35478809"],"doi":["10.1039/d1ra03615g"]}}