{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["66"],"submitter":["Su T"],"pubmed_abstract":["<h4>Background</h4>Resveratrol, a well-known natural compound and nutrient, activates the deacetylation ability of SIRT1, demonstrating p53-dependent apoptosis functions in many diseases. However, the nascent proteomic fluctuation caused by resveratrol is still unclear.<h4>Objective</h4>In this study, we investigated the effect of resveratrol on the nascent proteome and transcriptome initiated by SIRT1 activation, and we explored the mechanism of resveratrol in HEK 293T cells.<h4>Methods</h4>Bioorthogonal noncanonical amino acid tagging (BONCAT) is a method used to metabolically label nascent proteins. In this strategy, L-azidohomoalanine (AHA) was used to replace methionine (Met) under different conditions. Taking advantage of the click reaction between AHA and terminal alkyne- and disulf"],"journal":["Food & nutrition research"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9034730"],"repository":["biostudies-literature"],"pubmed_title":["Resveratrol regulates Hsp60 in HEK 293T cells during activation of SIRT1 revealed by nascent protein labeling strategy."],"pmcid":["PMC9034730"],"pubmed_authors":["Liu H","Su T","Hou Z","Zhang Z","Han X","Wang Z","Yang F"],"additional_accession":[]},"is_claimable":false,"name":"Resveratrol regulates Hsp60 in HEK 293T cells during activation of SIRT1 revealed by nascent protein labeling strategy.","description":"<h4>Background</h4>Resveratrol, a well-known natural compound and nutrient, activates the deacetylation ability of SIRT1, demonstrating p53-dependent apoptosis functions in many diseases. However, the nascent proteomic fluctuation caused by resveratrol is still unclear.<h4>Objective</h4>In this study, we investigated the effect of resveratrol on the nascent proteome and transcriptome initiated by SIRT1 activation, and we explored the mechanism of resveratrol in HEK 293T cells.<h4>Methods</h4>Bioorthogonal noncanonical amino acid tagging (BONCAT) is a method used to metabolically label nascent proteins. In this strategy, L-azidohomoalanine (AHA) was used to replace methionine (Met) under different conditions. Taking advantage of the click reaction between AHA and terminal alkyne- and disulf","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-05T11:07:04.208Z","creation":"2025-04-05T11:07:04.208Z"},"accession":"S-EPMC9034730","cross_references":{"pubmed":["35517847"],"doi":["10.29219/fnr.v66.8224"]}}