biostudies-literatureHerber CBNIA NIH HHSNIDDK NIH HHSNational Institutes of HealthNational Institute on Aging44https://www.ebi.ac.uk/biostudies/studies/S-EPMC9036729biostudies-literatureUnknown28(1)<h4>Background</h4>Menopausal hormone therapy (MHT) is recommended for only five years to treat vasomotor symptoms and vulvovaginal atrophy because of safety concerns with long-term treatment. We investigated the ability of 2',3',4'-trihydroxychalcone (2',3',4'-THC) to modulate estrogen receptor (ER)-mediated responses in order to find drug candidates that could potentially prevent the adverse effects of long-term MHT treatment.<h4>Methods</h4>Transfection assays, real time-polymerase chain reaction, and microarrays were used to evaluate the effects of 2',3',4'-THC on gene regulation. Radioligand binding studies were used to determine if 2',3',4'-THC binds to ERα. Cell proliferation was examined in MCF-7 breast cancer cells by using growth curves and flow cytometry. Western blots were usedMolecular medicine (Cambridge, Mass.)2',3',4'-Trihydroxychalcone changes estrogen receptor α regulation of genes and breast cancer cell proliferation by a reprogramming mechanism.PMC90367291 R43 DK109862-011 R43 AG072965-01R01 DK113019R43 AG072965R43 DK109862DK113019-01A1Yuan CChang AWang JCCohen ILeitman DCHerber CBfalse2',3',4'-Trihydroxychalcone changes estrogen receptor α regulation of genes and breast cancer cell proliferation by a reprogramming mechanism.<h4>Background</h4>Menopausal hormone therapy (MHT) is recommended for only five years to treat vasomotor symptoms and vulvovaginal atrophy because of safety concerns with long-term treatment. We investigated the ability of 2',3',4'-trihydroxychalcone (2',3',4'-THC) to modulate estrogen receptor (ER)-mediated responses in order to find drug candidates that could potentially prevent the adverse effects of long-term MHT treatment.<h4>Methods</h4>Transfection assays, real time-polymerase chain reaction, and microarrays were used to evaluate the effects of 2',3',4'-THC on gene regulation. Radioligand binding studies were used to determine if 2',3',4'-THC binds to ERα. Cell proliferation was examined in MCF-7 breast cancer cells by using growth curves and flow cytometry. Western blots were used2022-01-01T00:00:00Z2022 Apr2026-06-01T02:00:22.549Z2024-10-17T20:04:52.74ZS-EPMC90367293546871910.1186/s10020-022-00470-z