biostudies-literatureUnknown17(4)Pettifor A<h4>Background</h4>Early in the pandemic, transmission risk from asymptomatic infection was unclear, making it imperative to monitor infection in workplace settings. Further, data on SARS-CoV-2 seroprevalence within university populations has been limited.<h4>Methods</h4>We performed a longitudinal study of University research employees on campus July-December 2020. We conducted questionnaires on COVID-19 risk factors, RT-PCR testing, and SARS-CoV-2 serology using an in-house spike RBD assay, laboratory-based Spike NTD assay, and standard nucleocapsid platform assay. We estimated prevalence and cumulative incidence of seroconversion with 95% confidence intervals using the inverse of the Kaplan-Meier estimator.<h4>Results</h4>910 individuals were included in this analysis. At baseline, 6.2%PloS onee0267353https://www.ebi.ac.uk/biostudies/studies/S-EPMC9037948biostudies-literatureA prospective study of asymptomatic SARS-CoV-2 infection among individuals involved in academic research under limited operations during the COVID-19 pandemic.PMC9037948Maile RJadi RKuczynski KWallet SDittmer DSena ACTan Jde Silva AMJones CDAiello APremkumar LKim MShook-Sa BEPluta LDiPrete BLPettifor AWeber DJfalseA prospective study of asymptomatic SARS-CoV-2 infection among individuals involved in academic research under limited operations during the COVID-19 pandemic.<h4>Background</h4>Early in the pandemic, transmission risk from asymptomatic infection was unclear, making it imperative to monitor infection in workplace settings. Further, data on SARS-CoV-2 seroprevalence within university populations has been limited.<h4>Methods</h4>We performed a longitudinal study of University research employees on campus July-December 2020. We conducted questionnaires on COVID-19 risk factors, RT-PCR testing, and SARS-CoV-2 serology using an in-house spike RBD assay, laboratory-based Spike NTD assay, and standard nucleocapsid platform assay. We estimated prevalence and cumulative incidence of seroconversion with 95% confidence intervals using the inverse of the Kaplan-Meier estimator.<h4>Results</h4>910 individuals were included in this analysis. At baseline, 6.2%2022-01-01T00:00:00Z20222025-06-01T00:38:06.33Z2024-11-13T07:38:23.348ZS-EPMC90379483546815010.1371/journal.pone.0267353