{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Neubauer A"],"funding":["Swiss National Science Foundation"],"pagination":["e11456"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9039799"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(2)"],"pubmed_abstract":["<h4>Premise</h4>A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte-dominant life cycle and are critical to understanding the evolution of key land plant traits.<h4>Methods and results</h4>We describe a detailed protocol to isolate and transiently transform protoplasts of the model hornwort <i>Anthoceros agrestis</i>. The digestion of liquid cultures with Driselase yields a high number of viable protoplasts suitable for polyethylene glycol (PEG)-mediated transformation. We also report early signs of protoplast regeneration, such as chloroplast division and cell wall reconstitution.<h4>Conclusions</h4>This protoco"],"journal":["Applications in plant sciences"],"pubmed_title":["Step-by-step protocol for the isolation and transient transformation of hornwort protoplasts."],"pmcid":["PMC9039799"],"funding_grant_id":["184826","160004"],"pubmed_authors":["Notzold SI","Ruaud S","Neubauer A","Frangedakis E","Szovenyi P","Waller M","Li FW","Bailly A","Wicke S"],"additional_accession":[]},"is_claimable":false,"name":"Step-by-step protocol for the isolation and transient transformation of hornwort protoplasts.","description":"<h4>Premise</h4>A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte-dominant life cycle and are critical to understanding the evolution of key land plant traits.<h4>Methods and results</h4>We describe a detailed protocol to isolate and transiently transform protoplasts of the model hornwort <i>Anthoceros agrestis</i>. The digestion of liquid cultures with Driselase yields a high number of viable protoplasts suitable for polyethylene glycol (PEG)-mediated transformation. We also report early signs of protoplast regeneration, such as chloroplast division and cell wall reconstitution.<h4>Conclusions</h4>This protoco","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar-Apr","modification":"2025-04-26T15:12:51.312Z","creation":"2025-04-06T14:51:39.437Z"},"accession":"S-EPMC9039799","cross_references":{"pubmed":["35495192"],"doi":["10.1002/aps3.11456"]}}