{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramon-Sierra JM"],"funding":["Consejo Nacional de Ciencia y Tecnología","Tecnológico Nacional de México"],"pagination":["100177"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9039927"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Proteins from <i>Melipona beecheii</i> honey were purified by concanavalin A (conA) affinity chromatography and eluted with a stepwise glucose gradient into fractions named F2-F5. The conA-unbound fraction (F1) was further separated by molecular exclusion into fractions named MbF1-1,2 and MbF1-3. All fractions were evaluated for antibacterial activity against foodborne pathogens and antioxidant capacity. F1 fraction possessed highest antimicrobial activity against <i>S. aureus</i>, <i>L. monocytogenes</i>, <i>S.</i> Typhimurium, <i>E. coli</i> and <i>P. aeruginosa</i> with MIC's 1.4 ± 0.2, 15 ± 1, 39 ± 2, 1 ± 0.1, and 75 ± 2 µg/mL, respectively. F1, MbF1-1,2 and MbF1-3 had bactericidal effect except against <i>P. aeruginosa</i>. When the antioxidant capacity of the fractions was determined"],"journal":["Food chemistry: X"],"pubmed_title":["Antimicrobial and antioxidant activity of proteins isolated from <i>Melipona beecheii</i> honey."],"pmcid":["PMC9039927"],"funding_grant_id":["6748.18-P","221624"],"pubmed_authors":["Arias-Castro C","Rodriguez-Mendiola M","Ortiz-Vazquez E","Ramon-Sierra JM","Villanueva MA","Yam-Puc A"],"additional_accession":[]},"is_claimable":false,"name":"Antimicrobial and antioxidant activity of proteins isolated from <i>Melipona beecheii</i> honey.","description":"Proteins from <i>Melipona beecheii</i> honey were purified by concanavalin A (conA) affinity chromatography and eluted with a stepwise glucose gradient into fractions named F2-F5. The conA-unbound fraction (F1) was further separated by molecular exclusion into fractions named MbF1-1,2 and MbF1-3. All fractions were evaluated for antibacterial activity against foodborne pathogens and antioxidant capacity. F1 fraction possessed highest antimicrobial activity against <i>S. aureus</i>, <i>L. monocytogenes</i>, <i>S.</i> Typhimurium, <i>E. coli</i> and <i>P. aeruginosa</i> with MIC's 1.4 ± 0.2, 15 ± 1, 39 ± 2, 1 ± 0.1, and 75 ± 2 µg/mL, respectively. F1, MbF1-1,2 and MbF1-3 had bactericidal effect except against <i>P. aeruginosa</i>. When the antioxidant capacity of the fractions was determined","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-04T07:44:28.452Z","creation":"2025-04-04T07:44:28.452Z"},"accession":"S-EPMC9039927","cross_references":{"pubmed":["35498960"],"doi":["10.1016/j.fochx.2021.100177"]}}