biostudies-literatureRamon-Sierra JMConsejo Nacional de Ciencia y TecnologíaTecnológico Nacional de México100177https://www.ebi.ac.uk/biostudies/studies/S-EPMC9039927biostudies-literatureUnknown13Proteins from <i>Melipona beecheii</i> honey were purified by concanavalin A (conA) affinity chromatography and eluted with a stepwise glucose gradient into fractions named F2-F5. The conA-unbound fraction (F1) was further separated by molecular exclusion into fractions named MbF1-1,2 and MbF1-3. All fractions were evaluated for antibacterial activity against foodborne pathogens and antioxidant capacity. F1 fraction possessed highest antimicrobial activity against <i>S. aureus</i>, <i>L. monocytogenes</i>, <i>S.</i> Typhimurium, <i>E. coli</i> and <i>P. aeruginosa</i> with MIC's 1.4 ± 0.2, 15 ± 1, 39 ± 2, 1 ± 0.1, and 75 ± 2 µg/mL, respectively. F1, MbF1-1,2 and MbF1-3 had bactericidal effect except against <i>P. aeruginosa</i>. When the antioxidant capacity of the fractions was determinedFood chemistry: XAntimicrobial and antioxidant activity of proteins isolated from <i>Melipona beecheii</i> honey.PMC90399276748.18-P221624Arias-Castro CRodriguez-Mendiola MOrtiz-Vazquez ERamon-Sierra JMVillanueva MAYam-Puc AfalseAntimicrobial and antioxidant activity of proteins isolated from <i>Melipona beecheii</i> honey.Proteins from <i>Melipona beecheii</i> honey were purified by concanavalin A (conA) affinity chromatography and eluted with a stepwise glucose gradient into fractions named F2-F5. The conA-unbound fraction (F1) was further separated by molecular exclusion into fractions named MbF1-1,2 and MbF1-3. All fractions were evaluated for antibacterial activity against foodborne pathogens and antioxidant capacity. F1 fraction possessed highest antimicrobial activity against <i>S. aureus</i>, <i>L. monocytogenes</i>, <i>S.</i> Typhimurium, <i>E. coli</i> and <i>P. aeruginosa</i> with MIC's 1.4 ± 0.2, 15 ± 1, 39 ± 2, 1 ± 0.1, and 75 ± 2 µg/mL, respectively. F1, MbF1-1,2 and MbF1-3 had bactericidal effect except against <i>P. aeruginosa</i>. When the antioxidant capacity of the fractions was determined2022-01-01T00:00:00Z2022 Mar2025-04-04T07:44:28.452Z2025-04-04T07:44:28.452ZS-EPMC90399273549896010.1016/j.fochx.2021.100177