biostudies-literatureUnknown152Zurita-Cruz NDInstituto de Salud Carlos IIIMinisterio de Economía y CompetitividadEuropean Regional Development Fund<h4>Background</h4>SARS-CoV-2 viral load and kinetics assessed in serial blood samples from hospitalised COVID-19 patients by RT-PCR are poorly understood.<h4>Methods</h4>We conducted an observational, prospective case series study in hospitalised COVID-19 patients. Clinical outcome data (Intensive Care Unit admission and mortality) were collected from all patients until discharge. Viremia was determined longitudinally during hospitalisation, in plasma and serum samples collected sequentially, using two commercial and standardised RT-PCR techniques approved for use in diagnosis of SARS-CoV-2. Viral load (copies/mL and log10) was determined with quantitative TaqPath™COVID-19 test. Persistent viremia (PV) was defined as two or more consecutive quantifiable viral loads detected in blood samplJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology105166https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040491biostudies-literatureUsefulness of real-time RT-PCR to understand the kinetics of SARS-CoV-2 in blood: A prospective study.PMC9040491Gonzalez-Alvaro IMartin-Ramirez AZurita-Cruz NDRodriguez-Serrano DAFontan Garcia-Rodrigo LDe la Camara RCardenoso-Domingo LRoy-Vallejo EfalseUsefulness of real-time RT-PCR to understand the kinetics of SARS-CoV-2 in blood: A prospective study.<h4>Background</h4>SARS-CoV-2 viral load and kinetics assessed in serial blood samples from hospitalised COVID-19 patients by RT-PCR are poorly understood.<h4>Methods</h4>We conducted an observational, prospective case series study in hospitalised COVID-19 patients. Clinical outcome data (Intensive Care Unit admission and mortality) were collected from all patients until discharge. Viremia was determined longitudinally during hospitalisation, in plasma and serum samples collected sequentially, using two commercial and standardised RT-PCR techniques approved for use in diagnosis of SARS-CoV-2. Viral load (copies/mL and log10) was determined with quantitative TaqPath™COVID-19 test. Persistent viremia (PV) was defined as two or more consecutive quantifiable viral loads detected in blood sampl2022-01-01T00:00:00Z2022 Jul2026-07-16T20:08:39.519Z2024-11-14T01:55:48.258ZS-EPMC90404913559478410.1016/j.jcv.2022.105166