{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Anderson CE"],"funding":["National Science Foundation Graduate Research Fellowship Program","Department of Energy Biological and Environmental Research Award","Department of Energy Environmental Molecular Science Laboratory User Facility Award","UMN | Department of Bioproducts and Biosystems Engineering, University of Minnesota"],"pagination":["e0018822"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040566"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88(8)"],"pubmed_abstract":["Brown rot fungi dominate wood decomposition in coniferous forests, and their carbohydrate-selective mechanisms are of commercial interest. Brown rot was recently described as a two-step, sequential mechanism orchestrated by fungi using differentially expressed genes (DEGs) and consisting of oxidation via reactive oxygen species (ROS) followed by enzymatic saccharification. There have been indications, however, that the initial oxidation step itself might require induction. To capture this early gene regulation event, here, we integrated fine-scale cryosectioning with whole-transcriptome sequencing to dissect gene expression at the single-hyphal-cell scale (tens of micrometers). This improved the spatial resolution 50-fold, relative to previous work, and we were able to capture the activity"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Capturing an Early Gene Induction Event during Wood Decay by the Brown Rot Fungus <i>Rhodonia placenta</i>."],"pmcid":["PMC9040566"],"funding_grant_id":["CON-75851 project 00074041","EUP 50799","DE-SC0019427","Forest Worker Fellowship","Pawek Fellowship"],"pubmed_authors":["Anderson CE","Mitchell HD","Schilling JS","Zhang J","Chrisler WB","Orr G","Markillie LM","Gaffrey MJ"],"additional_accession":[]},"is_claimable":false,"name":"Capturing an Early Gene Induction Event during Wood Decay by the Brown Rot Fungus <i>Rhodonia placenta</i>.","description":"Brown rot fungi dominate wood decomposition in coniferous forests, and their carbohydrate-selective mechanisms are of commercial interest. Brown rot was recently described as a two-step, sequential mechanism orchestrated by fungi using differentially expressed genes (DEGs) and consisting of oxidation via reactive oxygen species (ROS) followed by enzymatic saccharification. There have been indications, however, that the initial oxidation step itself might require induction. To capture this early gene regulation event, here, we integrated fine-scale cryosectioning with whole-transcriptome sequencing to dissect gene expression at the single-hyphal-cell scale (tens of micrometers). This improved the spatial resolution 50-fold, relative to previous work, and we were able to capture the activity","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-06-07T03:18:29.616Z","creation":"2025-04-20T03:06:37.377Z"},"accession":"S-EPMC9040566","cross_references":{"pubmed":["35348388"],"doi":["10.1128/aem.00188-22"]}}