biostudies-literatureLi TNational Natural Science Foundation of ChinaNatural Science Foundation of Guangdong Province29376-29384https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040573biostudies-literatureUnknown11(47)Eight previously undescribed diarylheptanoids (1-8), together with fifteen known analogues (9-23), were isolated from the rhizomes of <i>Zingiber officinale</i>. Their structures were unambiguously determined by comprehensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. It is worth mentioning that 1-3 are the first reported structures of diaryl ether heptanoids in <i>Z. officinale</i>, whereas 15-17 were isolated from <i>Zingiber</i> for the first time. Furthermore, a gene enrichment analysis of the interacting targets indicated that diarylheptanoids were mainly associated with the anti-tumor activity by affecting DNA damage signaling pathway. The results showed that 6, 16-19 had remarkable inhibitory effects against five tumor cell lines (A549, HepG2, HeLa,RSC advancesDiarylheptanoid analogues from the rhizomes of <i>Zingiber officinale</i> and their anti-tumour activity.PMC90405732018A03031370781803347Pang QQZhou MYao XJLi HBLi TPan DBYao XSYu YfalseDiarylheptanoid analogues from the rhizomes of <i>Zingiber officinale</i> and their anti-tumour activity.Eight previously undescribed diarylheptanoids (1-8), together with fifteen known analogues (9-23), were isolated from the rhizomes of <i>Zingiber officinale</i>. Their structures were unambiguously determined by comprehensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. It is worth mentioning that 1-3 are the first reported structures of diaryl ether heptanoids in <i>Z. officinale</i>, whereas 15-17 were isolated from <i>Zingiber</i> for the first time. Furthermore, a gene enrichment analysis of the interacting targets indicated that diarylheptanoids were mainly associated with the anti-tumor activity by affecting DNA damage signaling pathway. The results showed that 6, 16-19 had remarkable inhibitory effects against five tumor cell lines (A549, HepG2, HeLa,2021-01-01T00:00:00Z2021 Sep2025-04-22T07:48:22.77Z2025-04-05T22:18:30.256ZS-EPMC90405733547956410.1039/d1ra03592d