biostudies-literatureBai SProgram for Changjiang Scholars Team in University of Ministry of Education of ChinaInnovation Team Project of Guangdong UniversityLocal Innovative and Research Teams Project of Guangdong Pearl River Talents Programe0245721https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040600biostudies-literatureUnknown88(8)We retrospectively investigated 326 samples that were collected from goose farms in Hainan Province, China, in 2017. A total of 33 carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates were identified from 326 samples, and the 33 CRKP isolates were characterized based on whole-genome sequencing (WGS) data from the Illumina and Oxford Nanopore Technologies (ONT) platforms. All of these 33 CRKP isolates possessed <i>bla</i>_NDM-5, and a single isolate coharbored <i>mcr-1</i> and <i>bla</i>_NDM-5, while 4 isolates carried multiple virulence and metal tolerance gene clusters. One CRKP strain (CMG-35-2) was selected for long sequence reading. A hybrid plasmid carrying the virulence, resistance, and metal resistance gene in the strain was found. It possessed 2 backbones Applied and environmental microbiologyMolecular Characteristics of Antimicrobial Resistance and Virulence in Klebsiella pneumoniae Strains Isolated from Goose Farms in Hainan, China.PMC90406002019KCXTD001IRT_17R392019BT02N054Wang MLi XLiu YBai SSun RLiao XKuang XSun JYu YfalseMolecular Characteristics of Antimicrobial Resistance and Virulence in Klebsiella pneumoniae Strains Isolated from Goose Farms in Hainan, China.We retrospectively investigated 326 samples that were collected from goose farms in Hainan Province, China, in 2017. A total of 33 carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates were identified from 326 samples, and the 33 CRKP isolates were characterized based on whole-genome sequencing (WGS) data from the Illumina and Oxford Nanopore Technologies (ONT) platforms. All of these 33 CRKP isolates possessed <i>bla</i>_NDM-5, and a single isolate coharbored <i>mcr-1</i> and <i>bla</i>_NDM-5, while 4 isolates carried multiple virulence and metal tolerance gene clusters. One CRKP strain (CMG-35-2) was selected for long sequence reading. A hybrid plasmid carrying the virulence, resistance, and metal resistance gene in the strain was found. It possessed 2 backbones 2022-01-01T00:00:00Z2022 Apr2026-06-07T07:33:31.346Z2025-02-19T01:28:05.681ZS-EPMC90406003538925210.1128/aem.02457-21