biostudies-literatureUnknown9Jia MSenecavirus A (SVA), an important member of the <i>Picornaviridae</i> family, causes vesicular disease in pigs. Here, we generated an EGFP-expressing recombinant SVA re-SVA-EGFP, which exhibited similar growth kinetics to its parental virus. The reporter SVA was used to study the role of pig ANTXR1 (pANTXR1) in SVA infection in a porcine alveolar macrophage cell line (PAM-Tang cells). Knockdown of the pANTXR1 significantly reduced SVA infection and replication in PAM-Tang cells, while re-expression of the pANTXR1 promoted the cell susceptibility to SVA infection. The results indicated that pANTXR1 is a crucial receptor mediating SVA infection. Subsequently, the viral endocytosis pathways for SVA entry into pig cells were investigated and the results showed that cholesterol played an essentFrontiers in veterinary science840655https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040607biostudies-literatureSenecavirus A Entry Into Host Cells Is Dependent on the Cholesterol-Mediated Endocytic Pathway.PMC9040607Meng FSun MZhang YYTang YDWang HJia MCai XfalseSenecavirus A Entry Into Host Cells Is Dependent on the Cholesterol-Mediated Endocytic Pathway.Senecavirus A (SVA), an important member of the <i>Picornaviridae</i> family, causes vesicular disease in pigs. Here, we generated an EGFP-expressing recombinant SVA re-SVA-EGFP, which exhibited similar growth kinetics to its parental virus. The reporter SVA was used to study the role of pig ANTXR1 (pANTXR1) in SVA infection in a porcine alveolar macrophage cell line (PAM-Tang cells). Knockdown of the pANTXR1 significantly reduced SVA infection and replication in PAM-Tang cells, while re-expression of the pANTXR1 promoted the cell susceptibility to SVA infection. The results indicated that pANTXR1 is a crucial receptor mediating SVA infection. Subsequently, the viral endocytosis pathways for SVA entry into pig cells were investigated and the results showed that cholesterol played an essent2022-01-01T00:00:00Z20222025-04-05T22:18:28.191Z2025-04-05T22:18:28.191ZS-EPMC90406073549872510.3389/fvets.2022.840655