{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(2)"],"submitter":["Valgepea K"],"pubmed_abstract":["Microbes that can recycle one-carbon (C<sub>1</sub>) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO<sub>2</sub> and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model acetogen Clostridium autoethanogenum grown autotrophically on three gas mixtures (CO, CO+H<sub>2</sub>, or CO+CO<sub>2</sub>+H<sub>2</sub>). We detect the prioritization of proteome allocation for C<sub>1</sub> fixation and the significant expression of proteins involved in the production of acetate and ethanol as well as proteins with"],"journal":["mSystems"],"pagination":["e0002622"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040625"],"repository":["biostudies-literature"],"pubmed_title":["Absolute Proteome Quantification in the Gas-Fermenting Acetogen <i>Clostridium autoethanogenum</i>."],"pmcid":["PMC9040625"],"pubmed_authors":["Mueller AP","Tappel R","Talbo G","Takemori N","Valgepea K","Marcellin E","Ludwig C","Kopke M","Takemori A","Mahamkali V","Nielsen LK","Simpson SD"],"additional_accession":[]},"is_claimable":false,"name":"Absolute Proteome Quantification in the Gas-Fermenting Acetogen <i>Clostridium autoethanogenum</i>.","description":"Microbes that can recycle one-carbon (C<sub>1</sub>) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO<sub>2</sub> and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model acetogen Clostridium autoethanogenum grown autotrophically on three gas mixtures (CO, CO+H<sub>2</sub>, or CO+CO<sub>2</sub>+H<sub>2</sub>). We detect the prioritization of proteome allocation for C<sub>1</sub> fixation and the significant expression of proteins involved in the production of acetate and ethanol as well as proteins with","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-05-31T12:58:54.468Z","creation":"2025-04-06T21:51:42.061Z"},"accession":"S-EPMC9040625","cross_references":{"pubmed":["35384696"],"doi":["10.1128/msystems.00026-22"]}}