biostudies-literatureUnknown7(2)Valgepea KMicrobes that can recycle one-carbon (C<sub>1</sub>) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO<sub>2</sub> and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model acetogen Clostridium autoethanogenum grown autotrophically on three gas mixtures (CO, CO+H<sub>2</sub>, or CO+CO<sub>2</sub>+H<sub>2</sub>). We detect the prioritization of proteome allocation for C<sub>1</sub> fixation and the significant expression of proteins involved in the production of acetate and ethanol as well as proteins withmSystemse0002622https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040625biostudies-literatureAbsolute Proteome Quantification in the Gas-Fermenting Acetogen <i>Clostridium autoethanogenum</i>.PMC9040625Mueller APTappel RTalbo GTakemori NValgepea KMarcellin ELudwig CKopke MTakemori AMahamkali VNielsen LKSimpson SDfalseAbsolute Proteome Quantification in the Gas-Fermenting Acetogen <i>Clostridium autoethanogenum</i>.Microbes that can recycle one-carbon (C<sub>1</sub>) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO<sub>2</sub> and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model acetogen Clostridium autoethanogenum grown autotrophically on three gas mixtures (CO, CO+H<sub>2</sub>, or CO+CO<sub>2</sub>+H<sub>2</sub>). We detect the prioritization of proteome allocation for C<sub>1</sub> fixation and the significant expression of proteins involved in the production of acetate and ethanol as well as proteins with2022-01-01T00:00:00Z2022 Apr2026-05-31T12:58:54.468Z2025-04-06T21:51:42.061ZS-EPMC90406253538469610.1128/msystems.00026-22