{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Qiu C"],"funding":["MOST | National Key Research and Development Program of China","Natural Science Foundation of Shandong Province","National Natural Science Foundation of China"],"pagination":["e0136621"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040634"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(2)"],"pubmed_abstract":["Malonyl-coenzyme A (malonyl-CoA) is an important precursor for producing various chemicals, but its low availability limits the synthesis of downstream products in Saccharomyces cerevisiae. Owing to the complexity of metabolism, evolutionary engineering is required for developing strains with improved malonyl-CoA synthesis. Here, using the biosensor we constructed previously, a growth-based screening system that links the availability of malonyl-CoA with cell growth is developed. Coupling this system with <i>in vivo</i> continuous mutagenesis enabled rapid generation of genome-scale mutation library and screening strains with improved malonyl-CoA availability. The mutant strains are analyzed by whole-genome sequencing and transcriptome analysis. The omics analysis revealed that the carbon "],"journal":["mSystems"],"pubmed_title":["Biosensor-Coupled <i>In Vivo</i> Mutagenesis and Omics Analysis Reveals Reduced Lysine and Arginine Synthesis To Improve Malonyl-Coenzyme A Flux in <i>Saccharomyces cerevisiae</i>."],"pmcid":["PMC9040634"],"funding_grant_id":["ZR2020YQ18","31770101","2019YFA0904900","31970082"],"pubmed_authors":["Hou J","Qiu C","Tao H","Bao X","Huang M","Shen Y","Hou Y","Zhao J","Qi Q"],"additional_accession":[]},"is_claimable":false,"name":"Biosensor-Coupled <i>In Vivo</i> Mutagenesis and Omics Analysis Reveals Reduced Lysine and Arginine Synthesis To Improve Malonyl-Coenzyme A Flux in <i>Saccharomyces cerevisiae</i>.","description":"Malonyl-coenzyme A (malonyl-CoA) is an important precursor for producing various chemicals, but its low availability limits the synthesis of downstream products in Saccharomyces cerevisiae. Owing to the complexity of metabolism, evolutionary engineering is required for developing strains with improved malonyl-CoA synthesis. Here, using the biosensor we constructed previously, a growth-based screening system that links the availability of malonyl-CoA with cell growth is developed. Coupling this system with <i>in vivo</i> continuous mutagenesis enabled rapid generation of genome-scale mutation library and screening strains with improved malonyl-CoA availability. The mutant strains are analyzed by whole-genome sequencing and transcriptome analysis. The omics analysis revealed that the carbon ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-06-12T09:07:25.098Z","creation":"2024-11-07T10:59:23.129Z"},"accession":"S-EPMC9040634","cross_references":{"pubmed":["35229648"],"doi":["10.1128/msystems.01366-21"]}}