biostudies-literatureUnknown12(20)Zhang SZinc finger antiviral proteins (ZAP) can significantly inhibit the replication of avian leukosis virus subgroup J (ALV-J), but the traditional method of ZAP administration is by injection, which can easily cause stress effects in chickens. In this work, we established a sodium alginate/atractylodis macrocephalae system for the encapsulation of CCCH-type zinc finger antiviral protein (CCCH-ZAP). Because of the high cost of ZAP, we first chose bovine serum albumin (BSA) as a model protein to investigate the encapsulation performance. The SEM images clearly confirmed that BSA and the sodium alginate/atractylodis macrocephalae system can assemble easily to form relatively stable nanostructures, and the encapsulation amount of BSA can reach 68%. Subsequently, the encapsulation of ZAP was studieRSC advances12600-12606https://www.ebi.ac.uk/biostudies/studies/S-EPMC9040642biostudies-literatureAssembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system.PMC9040642Li YFan HYang KYi CLiu SZhang SSun JCheng ZfalseAssembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system.Zinc finger antiviral proteins (ZAP) can significantly inhibit the replication of avian leukosis virus subgroup J (ALV-J), but the traditional method of ZAP administration is by injection, which can easily cause stress effects in chickens. In this work, we established a sodium alginate/atractylodis macrocephalae system for the encapsulation of CCCH-type zinc finger antiviral protein (CCCH-ZAP). Because of the high cost of ZAP, we first chose bovine serum albumin (BSA) as a model protein to investigate the encapsulation performance. The SEM images clearly confirmed that BSA and the sodium alginate/atractylodis macrocephalae system can assemble easily to form relatively stable nanostructures, and the encapsulation amount of BSA can reach 68%. Subsequently, the encapsulation of ZAP was studie2022-01-01T00:00:00Z2022 Apr2025-04-22T07:50:00.604Z2025-04-05T22:16:46.727ZS-EPMC90406423548036310.1039/d2ra01767a